Visualization of prion infection in transgenic mice expressing green fluorescent protein-tagged prion protein

被引:38
作者
Barmada, SJ [1 ]
Harris, DA [1 ]
机构
[1] Washington Univ, Sch Med, Dept Cell Biol & Physiol, St Louis, MO 63110 USA
关键词
prion; scrapie; transgenic; green fluorescent protein; neurodegeneration; Golgi;
D O I
10.1523/JNEUROSCI.1192-05.2005
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
Tg(PrP-EGFP) mice express an enhanced green fluorescent protein (EGFP)-tagged version of the prion protein ( PrP) that behaves like endogenous PrP in terms of its posttranslational processing, anatomical localization, and functional activity. In this study, we describe experiments in which Tg(PrP-EGFP) mice were inoculated intracerebrally with scrapie prions. Although PrP-EGFP was incapable of sustaining prion infection in Tg(PrP-EGFP)/Prn-p(0/0) mice, it acted as a dominant-negative inhibitor that bound to, and fluorescently marked, deposits of PrPSc generated from endogenous PrP in Tg(PrP-EGFP)/Prn-p(0/0) mice. Scrapie infection of these latter animals caused a progressive accumulation of fluorescent PrP-EGFP aggregates in neuropil, axons, and prominently in the Golgi apparatus of neurons. Our results provide an entirely new picture of PrPSc localization during the course of prion infection, and they identify for the first time intracellular sites of PrPSc formation that are not well visualized with conventional immunohistochemical techniques.
引用
收藏
页码:5824 / 5832
页数:9
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