The use of oxygen uptake rate measurements to control the supply of toxic substrate:: toluene hydroxylation by Pseudomonas putida UV4

被引：20

作者：

Carragher, JM

McClean, WS

Woodley, JM

Hack, CJ ^{[1
]}

机构：

[1] Univ Ulster, Biotechnol Res Grp, Coleraine BT52 1SA, Londonderry, North Ireland

[2] UCL, Dept Biochem Engn, Adv Ctr Biochem Engn, London WC1E 7JE, England

来源：

ENZYME AND MICROBIAL TECHNOLOGY | 2001年 / 28卷 / 2-3期

关键词：

fed-batch biocatalysis; toxic; poorly water-soluble substrate; oxygen uptake rate; feed-back control; hydroxylation; Pseudomonas putida;

D O I：

10.1016/S0141-0229(00)00289-1

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

During toluene hydroxylation, catalyzed by Pseudomonas putida UV4 one molecule of oxygen is added to the aromatic ring to produce the dihydroxylated (non-aromatic) ring structure, toluene cis-glycol. Toluene, which is toxic to the cells at aqueous phase concentration above (similar to2.4 mmol), is fed to the reactor. A feed-back control system based on oxygen uptake rate measurements was used to control the feed rate, and thus maintain the aqueous phase toluene concentration in the desired range for zero order kinetics. (C) 2001 Elsevier Science Inc. All rights reserved.

引用

页码：183 / 188

页数：6