Antioxidative activity of the hydrolytic enzyme treated Sorbus commixta Hedl. and its inhibitory effect on matrix metalloproteinase-1 in UV irradiated human dermal fibroblasts

被引:29
作者
Bae, Jun-Tae
Sim, Gwan-Sub
Kim, Jin-Hwa
Pyo, Hyeong-Bae
Yun, Jong-Won
Lee, Bum-Chun
机构
[1] Hanbul Cosmet Co Ltd, R&D Ctr, Chungbuk 369830, South Korea
[2] Daegu Univ, Dept Biotechnol, Kyongsan 712714, South Korea
关键词
Sorbus commixta Hedl; hydrolytic enzymes; antioxidant activity; matrix metalloproteinase-1; human dermal fibroblasts;
D O I
10.1007/BF02980246
中图分类号
R914 [药物化学];
学科分类号
100701 [药物化学];
摘要
Superoxide radical. scavenging activity and DPPH radical scavenging activity were assessed in order to evaluate the antioxidant effect of the Sorbus commixta Hedl. extract (SCoE). SCoE was also treated with several carbohydrate-hydrolytic enzymes that significantly increased the total phenol and flavonoid composition of SCoE. The enzymatically treated SCoE was then assessed for antioxidative activity. The most efficient radical scavenging activity was observed when SCoE was treated with -glucanase. The radical scavenging activity of beta-glucanase-treated SCoE (beta-GSCoE) enhanced the viability of human dermal fibroblasts (HDFs) exposed to ultraviolet (UV) light. The intracellular reactive oxygen species (ROS) scavenging activity of beta-GSCoE was assessed using UVB (20 mJ/cm(2))-irradiated HDFs. UVB irradiation increased dichlorofluorescein (DCF) fluorescence, which was measured by a 5-(6-)chloromethyl-2',7'dichlorodihydrofluorescein diacetate (CM-H(2)DCFDA). DCF-fluorescence was significantly decreased in the beta-GSCoE-containing culture medium, suggesting that beta-GSCoE scavenges free radicals. The protective effect was further verified by assessing the expression of matrix meialloproteinase-1 (MMP-1) in UVA-irradiated HDFs. The treatment of UVA-irradiated HDFs with beta-GSCoE resulted in a dose-dependent decrease in the expression level of MMP-1 protein and mRNA. These results suggest that beta-GSCoE may mitigate the effects of photoaging in skin by reducing UV-induced adverse skin reactions.
引用
收藏
页码:1116 / 1123
页数:8
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