Dissociating the enhancing and inhibitory effects of pertussis toxin on autoimmune disease

被引：9

作者：

Su, SB ^{[1
]}

Silver, PB ^{[1
]}

Wang, P ^{[1
]}

Chan, CC ^{[1
]}

Caspi, RR ^{[1
]}

机构：

[1] NEI, Immunol Lab, NIH, Bethesda, MD 20892 USA

来源：

JOURNAL OF IMMUNOLOGY | 2003年 / 171卷 / 05期

关键词：

D O I：

10.4049/jimmunol.171.5.2314

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Pertussis toxin (PT) has both enhancing and inhibitory effects on experimental autoimmune disease, depending on its. time of administration relative to immunization. The inhibitory effect is due to blocking of G(i)-coupled receptors by the enzymatic A subunit. In this study, we attribute the enhancing effect of PT to the cell-binding B subunit (PT-B). C57BL/6 mice, a strain that requires PT to develop experimental uveitis, were immunized with a retinal Ag and were injected with whole PT, PT-B, or vehicle. Disease and associated immunological responses were evaluated. The results showed that PT-B, determined to be free of biologically significant contamination with whole PT or with endotoxin, was able to mimic all the effects of PT with respect to disease induction, enhancement of delayed-type hypersensitivity, enhancement of lymphocyte proliferation, induction of an innate IL-12 response, and promotion of an adaptive IFN-gamma response to the uveitogenic Ag. Our results suggest that PT-B is largely responsible for the disease-enhancing properties of PT.

引用

页码：2314 / 2319

页数：6

共 32 条

[1] Pertussis toxin alters the innate and the adaptive immune responses in a pertussis-dependent model of autoimmunity [J].