Primers of glycosaminoglycan biosynthesis from Peruvian Rain Forest plants

被引:9
作者
Taylor, WH
Sinha, A
Khan, IA
McDaniel, ST
Esko, JD [1 ]
机构
[1] Univ Calif San Diego, Dept Med, Div Cellular & Mol Med, La Jolla, CA 92093 USA
[2] Univ Calif San Diego, Glycobiol Program, La Jolla, CA 92093 USA
[3] Univ Mississippi, Pharmaceut Sci Res Inst, Natl Ctr Dev Nat Prod, University, MS 38677 USA
[4] Mississippi State Univ, Dept Biol Sci, Inst Bot Explorat, Mississippi State, MS 39762 USA
关键词
D O I
10.1074/jbc.273.35.22260
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We have developed a rapid, high throughput screening assay for compounds that alter the assembly of glycosaminoglycan chains in Chinese hamster ovary cells. The assay uses autoradiography to measure the binding of newly synthesized [S-35]proteoglycans and [S-35]glycosaminoglycans to a positively charged membrane. Screening over 1000 extracts from a random plant collection obtained from the Amazon rain forest yielded five plants that stimulated glycosaminoglycan assembly in both wild-type cells and a mutant cell line defective in xylosyltransferase (the first committed enzyme involved in glycosaminoglycan biosynthesis). Fractionation of an extract of Maieta guianensis by silica gel and reverse-phase chromatography yielded two pure compounds with stimulatory activity. Spectroscopic analysis by NMR and mass spectrometry revealed that the active principles were xylosides of dimethylated ellagic acid. One of the compounds also contained a galloyl group at C-3 of the xylose moiety. These findings suggest that plants and other natural products may be a source of agents that can potentially alter glycosaminoglycan and proteoglycan formation in animal cells.
引用
收藏
页码:22260 / 22266
页数:7
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