Glycoside hydrolases as components of putative carbohydrate biosensor proteins in Clostridium thermocellum

被引:39
作者
Bahari, Liat [1 ]
Gilad, Yuval [1 ]
Borovok, Ilya [2 ]
Kahel-Raifer, Hamutal [2 ]
Dassa, Bareket [1 ]
Nataf, Yakir [3 ]
Shoham, Yuval [3 ]
Lamed, Raphael [2 ]
Bayer, Edward A. [1 ]
机构
[1] Weizmann Inst Sci, Dept Biol Chem, IL-76100 Rehovot, Israel
[2] Tel Aviv Univ, George S Wise Fac Life Sci, Dept Mol Microbiol & Biotechnol, IL-69978 Tel Aviv, Israel
[3] Technion Israel Inst Technol, Dept Biotechnol & Food Engn, IL-32000 Haifa, Israel
基金
以色列科学基金会;
关键词
Anti-sigma factor; RsgI; Family 24 sigma factor; Carbohydrate-binding module (CBM); Glycoside hydrolase (GH); QUANTITATIVE PROTEOMIC ANALYSIS; CELLULOMONAS-FIMI; BINDING DOMAINS; FAMILIES; SEQUENCE; XYLANASE; CELLULOSOME; DATABASE; CLASSIFICATION; ARABINOXYLANS;
D O I
10.1007/s10295-010-0848-9
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The composition of the cellulase system in the cellulosome-producing bacterium, Clostridium thermocellum, has been reported to change in response to growth on different carbon sources. Recently, an extensive carbohydrate-sensing mechanism, purported to regulate the activation of genes coding for polysaccharide-degrading enzymes, was suggested. In this system, CBM modules, comprising extracellular components of RsgI-like anti-sigma factors, were proposed to function as carbohydrate sensors, through which a set of cellulose utilization genes are activated by the associated sigma(I)-like factors. An extracellular module of one of these RsgI-like proteins (Cthe_2119) was annotated as a family 10 glycoside hydrolase, RsgI6-GH10, and a second putative anti-sigma factor (Cthe_1471), related in sequence to Rsi24, was found to contain a module that resembles a family 5 glycoside hydrolase (termed herein Rsi24C-GH5). The present study examines the relevance of these two glycoside hydrolases as sensors in this signal-transmission system. The RsgI6-GH10 was found to bind xylan matrices but exhibited low enzymatic activity on this substrate. In addition, this glycoside hydrolase module was shown to interact with crystalline cellulose although no hydrolytic activity was detected on cellulosic substrates. Bioinformatic analysis of the Rsi24C-GH5 showed a glutamate-to-glutamine substitution that would presumably preclude catalytic activity. Indeed, the recombinant module was shown to bind to cellulose, but showed no hydrolytic activity. These observations suggest that these two glycoside hydrolases underwent an evolutionary adaptation to function as polysaccharide binding agents rather than enzymatic components and thus serve in the capacity of extracellular carbohydrate sensors.
引用
收藏
页码:825 / 832
页数:8
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