The protective effect of magnesium lithospermate B against glucose-induced intracellular oxidative damage

被引：31

作者：

Qu, Jian ^{[1
]}

Ren, Xian ^{[2
]}

Hou, Rui-ying ^{[1
]}

Dai, Xing-ping ^{[1
]}

Zhao, Ying-chun ^{[3
]}

Xu, Xiao-jing ^{[1
]}

Zhang, Wei ^{[1
]}

Zhou, Gan ^{[1
]}

Zhou, Hong-hao ^{[1
]}

Liu, Zhao-qian ^{[1
]}

机构：

[1] Cent S Univ, Inst Clin Pharmacol, Hunan Key Lab Pharmacogenet, Xiangya Sch Med, Changsha 410078, Hunan, Peoples R China

[2] Shanghai Green Valley Pharmaceut Co Ltd, Shanghai 201304, Peoples R China

[3] Creighton Univ, Med Ctr, Labs Funct Genom & Prote, Omaha, NE 68131 USA

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2011年 / 411卷 / 01期

基金：

中国国家自然科学基金;

关键词：

Magnesium lithospermate B; Reative oxygen species; HO-1; Nrf2; HEME OXYGENASE-1; CELLS;

D O I：

10.1016/j.bbrc.2011.06.071

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

Objectives: To investigate the effects of magnesium lithospermate B (LAB) on intracellular reactive oxygen species (ROS) production induced by high dose of glucose or H2O2, we explored the influences of LAB on the expression of heme oxygenase-1 (HO-1) and nuclear factor E2-related factor-2 (Nrf2) in HEK293T cells after treatment with high dose of glucose. Materials and methods: The total nuclear proteins in HEK293T cells were extracted with Cytoplasmic Protein Extraction Kit. The ROS level was determined by flow cytometry. The mRNA and protein expression of HO-1 and Nrf2 were determined by quantitative reverse transcription polymerase chain reaction (qRT-PCR) and Western blot. Results: LAB reduced the ROS production in HEK293T cells cultured under oxidative stress. High dose of glucose enhanced the expression of HO-1 mRNA and HO-1 protein in a time-dependent manner. LAB enhanced the expression of HO-1 mRNA and HO-1 protein in a dose-dependent manner treated with high dose of glucose. The amount of Nrf2 translocation was enhanced after cells were pretreated with 50 mu mol/L or 100 mu mol/L LAB. Silencing of Nrf2 gene eliminated the enhanced expression of HO-1 protein induced by high dose of glucose plus LAB. Conclusions: LAB plays an important role against glucose-induced intracellular oxidative damage. The enhanced expression of HO-1 mRNA and HO-1 protein caused by LAB is regulated via Nrf2 signal pathway. Crown Copyright (C) 2011 Published by Elsevier Inc. All rights reserved.

引用

页码：32 / 39

页数：8

共 21 条

[1]

IDENTIFICATION OF A 2ND REGION UPSTREAM OF THE MOUSE HEME OXYGENASE-1 GENE THAT FUNCTIONS AS A BASAL LEVEL AND INDUCER-DEPENDENT TRANSCRIPTION ENHANCER [J].