MR tracking of transplanted cells with "positive contrast" using manganese oxide nanoparticles

被引：131

作者：

Gilad, Assaf A. ^{[1
,2
]}

Walczak, Piotr ^{[1
,2
]}

McMahon, Michael T. ^{[1
,3
]}

Na, Hyon Bin ^{[4
,5
]}

Lee, Jung Hee ^{[6
]}

An, Kwangjin ^{[4
,5
]}

Hyeon, Taegwhan ^{[4
,5
]}

van Zijl, Peter C. M. ^{[1
,3
]}

Bulte, Jeff W. M. ^{[1
,2
,7
,8
]}

机构：

[1] Johns Hopkins Univ, Sch Med, Russell H Morgan Dept Radiol & Radiol Sci, Div MR Res, Baltimore, MD 21205 USA

[2] Johns Hopkins Univ, Sch Med, Cell Imaging Sect, Inst Cell Engn, Baltimore, MD 21205 USA

[3] Kennedy Krieger Inst, FM Kirby Ctr Funct Brain Imaging, Baltimore, MD USA

[4] Seoul Natl Univ, Natl Creat Res Initiat, Ctr Oxide Nanocrystalline Mat, Seoul, South Korea

[5] Seoul Natl Univ, Sch Chem & Biol Engn, Seoul, South Korea

[6] Sungkyunkwan Univ, Sch Med, Samsung Med Ctr, Dept Radiol, Seoul, South Korea

[7] Johns Hopkins Univ, Whiting Sch Engn, Dept Chem & Biomol Engn, Baltimore, MD 21205 USA

[8] Johns Hopkins Univ, Sch Med, Dept Biomed Engn, Baltimore, MD 21205 USA

来源：

MAGNETIC RESONANCE IN MEDICINE | 2008年 / 60卷 / 01期

关键词：

manganese oxide; iron oxide; cellular imaging; contrast agent; transplantation; nanoparticles;

D O I：

10.1002/mrm.21622

中图分类号：

R8 [特种医学]; R445 [影像诊断学];

学科分类号：

1002 ; 100207 ; 1009 ;

摘要：

Rat glioma cells were labeled using electroporation with either manganese oxide (MnO) or superparamagnetic iron oxide (SPIO) nanoparticles. The viability and proliferation of SPIO-labeled cells (1.9 mg Fe/ml) or cells electroporated with a low dose of MnO (100 mu g Mn/ml) was not significantly different from unlabeled cells; a higher MnO dose (785 mu g Mn/ml) was found to be toxic. The cellular ion content was 0.1-0.3 pg Mn/cell and 4.4 pg Fe/cell, respectively, with cellular relaxivities of 2.5-4.8 s(-1) (RI) and 45-84 s(-1) (R-2) for MnO-labeled cells. Labeled cells (SPIO and low-dose MnO) were each transplanted in contralateral brain hemispheres of rats and imaged in vivo at 9.4T. While SPIO-labeled cells produced a strong "negative contrast" due to the increase in R-2, MnO-Iabeled cells produced "positive contrast" with an increased R-1. Simultaneous imaging of both transplants with opposite contrast offers a method for MR "double labeling" of different cell populations.

引用

页码：1 / 7

页数：7

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