Inhibition of phospholipase C gamma 1 activity by amentoflavone isolated from Selaginella tamariscina

被引：49

作者：

Lee, HS ^{[1
]}

Oh, WK ^{[1
]}

Kim, BY ^{[1
]}

Ahn, SC ^{[1
]}

Kang, DO ^{[1
]}

Shin, DI ^{[1
]}

Kim, J ^{[1
]}

Mheen, T ^{[1
]}

Ahn, JS ^{[1
]}

机构：

[1] SEOUL NATL UNIV, COLL PHARM, SEOUL 151742, SOUTH KOREA

来源：

PLANTA MEDICA | 1996年 / 62卷 / 04期

关键词：

phospholipase C gamma 1 inhibitor; phosphoinositides (PI)-turnover; amentoflavone; Selaginella tamariscina; Selaginellaceae; Kampo medicines;

D O I：

10.1055/s-2006-957887

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Amentoflavone was isolated as an inhibitor of phospholipase C gamma 1 (PLC gamma 1) and phosphoinositides (PI)-turnover in PLC gamma 1 overexpressing NIH3T3 fibroblasts (NIH3T3 gamma 1) from Selaginella tamariscina (Selaginellaceae) together with other related biflavonoids, isocryptomerin and cryptomerin B. Only amentoflavone inhibited the PLC gamma 1 activity with an IC50 of 29 mu M and the formation of total inositol phosphates (IPt) in PDGF-stimulated NIH3T3 gamma 1 with an IC50 of 9.2 mu M but did not show inhibitory activity against protein kinase C. Isocryptomerin and cryptomerin B did not show inhibitory activity against PLC gamma 1 at the concentration of 1 50 mu M, and did not inhibit IPt production in PDGF-induced NIH3T3 gamma 1 at the concentration of 180 mu M.

引用

页码：293 / 296

页数：4

共 22 条

[1] STRUCTURE OF A NOVEL PHOSPHOLIPASE-C INHIBITOR, VINAXANTHONE (RO-09-1450), PRODUCED BY PENICILLIUM-VINACEUM [J].