Rhamnogalacturonan II, a dominant polysaccharide in juices produced by enzymic liquefaction of fruits and vegetables

被引：69

作者：

Doco, T ^{[1
]}

Williams, P ^{[1
]}

Vidal, S ^{[1
]}

Pellerin, P ^{[1
]}

机构：

[1] INRA,INST PROD VIGNE,UNITE RECH POLYMERES & TECH PHYSICOCHIM,F-34060 MONTPELLIER,FRANCE

来源：

CARBOHYDRATE RESEARCH | 1997年 / 297卷 / 02期

关键词：

Rhamnogalacturonan II; apple; carrot; tomato; pectin; pectic polysaccharide; Malus domestica; Solanum lycopersicum; Daucus carota; enzymic liquefaction; pectinases;

D O I：

10.1016/S0008-6215(96)00260-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Rhamnogalacturonan II (RG-II), a small complex pectic polysaccharide, is released from apple (Malus domestica), carrot (Daucus carota), and tomato (Solanum lycopersicum) by treatment with two commercial liquefying enzyme preparations. RG-II was isolated by size-exclusion chromatography from apple, tomato, and carrot juices obtained by enzymic liquefaction. All the RG-IIs contained the diagnostic sugars, apiose, 2-O-methyl-L-fucose 2-O-methyl-D-xylose, aceric acid, Kdo and Dha. Glycosyl-linkage compositions of the neutral and acidic sugars, including aceric acid, were consistent with the hypothetical model described for sycamore RG-II confirming the conservation of RG-II in plants. Thus, when pectinolytic enzyme preparations are used to process fruits and vegetables, RG-II is released as a main soluble polysaccharide fraction while other pectic polysaccharides are heavily degraded. (C) 1997 Elsevier Science Ltd.

引用

页码：181 / 186

页数：6