The interaction of DNA mismatch repair proteins with human exonuclease I

被引：128

作者：

Schmutte, C ^{[1
]}

Sadoff, MM ^{[1
]}

Shim, KS ^{[1
]}

Acharya, S ^{[1
]}

Fishel, R ^{[1
]}

机构：

[1] Thomas Jefferson Univ, Kimmel Canc Ctr, Genet & Mol Biol Program, Dept Microbiol & Immunol, Philadelphia, PA 19107 USA

来源：

JOURNAL OF BIOLOGICAL CHEMISTRY | 2001年 / 276卷 / 35期

关键词：

D O I：

10.1074/jbc.M102670200

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Exonucleolytic degradation of DNA is an essential part of many DNA metabolic processes including DNA mismatch repair (MMR) and recombination. Human exonuclease I (hExoI) is a member of a family of conserved 5' --> 3' exonucleases, which are implicated in these processes by genetic studies. Here, we demonstrate that hExoI binds strongly to hMLH1, and we describe interaction regions between hExoI and the MMR proteins hMSH2, hMSH3, and hMLH1. In addition, hExoI forms an immunoprecipitable complex with hMLH1/hPMS2 in vivo. The study of interaction regions suggests a biochemical mechanism of the involvement of hExoI as a downstream effector in MMR and/or DNA recombination.

引用

页码：33011 / 33018

页数：8

共 61 条

[51] Identification and characterization of Saccharomyces cerevisiae EXO1, a gene encoding an exonuclease that interacts with MSH2
Tishkoff, DX
Boerger, AL
Bertrand, P
Filosi, N
Gaida, GM
Kane, MF
Kolodner, RD
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1997, 94 (14) : 7487 - 7492
[52] Tran HT, 1999, MOL CELL BIOL, V19, P2000
[53] Functional studies on the candidate ATPase domains of Saccharomyces cerevisiae MutLα
Tran, PT
Liskay, RM
[J]. MOLECULAR AND CELLULAR BIOLOGY, 2000, 20 (17) : 6390 - 6398
[54] Viswanathan M, 1998, GENETICS, V149, P7
[55] Functional specificity of MutL homologs in yeast: Evidence for three Mlh1-based heterocomplexes with distinct roles during meiosis in recombination and mismatch correction
Wang, TF
Kleckner, N
Hunter, N
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1999, 96 (24) : 13914 - 13919
[56] Mechanisms of inactivation of mismatch repair genes in human colorectal cancer cell lines: The predominant role of hMLH1
Wheeler, JMD
Beck, NE
Kim, HC
Tomlinson, IPM
Mortensen, NJM
Bodmer, WF
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1999, 96 (18) : 10296 - 10301
[57] Hex1: a new human Rad2 nuclease family member with homology to yeast exonuclease 1
Wilson, DM
Carney, JP
Coleman, MA
Adamson, AW
Christensen, M
Lamerdin, JE
[J]. NUCLEIC ACIDS RESEARCH, 1998, 26 (16) : 3762 - 3768
[58] Dissociation of mismatch recognition and ATPase activity by hMSH2-hMSH3
Wilson, T
Guerrette, S
Fishel, R
[J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 1999, 274 (31) : 21659 - 21664
[59] Human DNA repair genes
Wood, RD
Mitchell, M
Sgouros, J
Lindahl, T
[J]. SCIENCE, 2001, 291 (5507) : 1284 - +
[60] Deletion mutation analysis of the mutS gene in Escherichia coli
Wu, TH
Marinus, MG
[J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 1999, 274 (09) : 5948 - 5952

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