Different PrlA proteins increase the efficiency of periplasmic production of human interleukin-6 in Escherichia coli

被引：8

作者：

PerezPerez, J ^{[1
]}

Barbero, JL ^{[1
]}

Marquez, G ^{[1
]}

Gutierrez, J ^{[1
]}

机构：

[1] PHARMACIA & UPJOHN INC,DEPT INVEST,MADRID,SPAIN

来源：

JOURNAL OF BIOTECHNOLOGY | 1996年 / 49卷 / 1-3期

关键词：

protein secretion; interleukin-6; secY;

D O I：

10.1016/0168-1656(96)83990-3

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The export efficiency of a fusion of the Escherichia coli preOmpA signal peptide to human interleukin-6 can be significantly raised by coexpressing three different prlA alleles of secY along with wild type secE. The effect seems prlA-specific, as prlGl (a prl allele of secE) does not affect the export of preOmpA-hIL-6. Coexpression of secD and secF also stimulates the export of the fusion protein.

引用

收藏

页码：245 / 247

页数：3

相关论文

共 10 条

[1] TOPOLOGY ANALYSIS OF THE SECY PROTEIN, AN INTEGRAL MEMBRANE-PROTEIN INVOLVED IN PROTEIN EXPORT IN ESCHERICHIA-COLI [J].

AKIYAMA, Y ;

ITO, K .

EMBO JOURNAL, 1987, 6 (11) :3465-3470

[2] SECD AND SECF ARE REQUIRED FOR THE PROTON ELECTROCHEMICAL GRADIENT STIMULATION OF PREPROTEIN TRANSLOCATION [J].

ARKOWITZ, RA ;

WICKNER, W .

EMBO JOURNAL, 1994, 13 (04) :954-963

[3] PRODUCTION AND SECRETION OF HUMAN INTERLEUKIN-6 INTO THE PERIPLASM OF ESCHERICHIA-COLI - EFFICIENT PROCESSING OF N-TERMINAL VARIANTS OF HIL6 BY THE ESCHERICHIA-COLI SIGNAL PEPTIDASE [J].

BARTHELEMY, I ;

DEBUITRAGO, GG ;

CARREIRO, C ;

RONCAL, F ;

PEREZARANDA, A ;

MARQUEZ, G ;

BARBERO, JL .

JOURNAL OF BIOTECHNOLOGY, 1993, 27 (03) :307-316

[4] PRLA (SECY) AND PRLG (SECE) INTERACT DIRECTLY AND FUNCTION SEQUENTIALLY DURING PROTEIN TRANSLOCATION IN ESCHERICHIA-COLI [J].

BIEKER, KL ;

SILHAVY, TJ .

CELL, 1990, 61 (05) :833-842

[5] THE ALLELE-SPECIFIC SYNTHETIC LETHALITY OF PRLA-PRLG DOUBLE MUTANTS PREDICTS INTERACTIVE DOMAINS OF SECY AND SECE [J].

FLOWER, AM ;

OSBORNE, RS ;

SILHAVY, TJ .

EMBO JOURNAL, 1995, 14 (05) :884-893

[6] INCREASING THE EFFICIENCY OF PROTEIN EXPORT IN ESCHERICHIA-COLI [J].

PEREZPEREZ, J ;

MARQUEZ, G ;

BARBERO, JL ;

GUTIERREZ, J .

BIO-TECHNOLOGY, 1994, 12 (02) :178-180

[7] AN ARABINOSE-INDUCIBLE EXPRESSION VECTOR, PAR3, COMPATIBLE WITH COLE1-DERIVED PLASMIDS [J].

PEREZPEREZ, J ;

GUTIERREZ, J .

GENE, 1995, 158 (01) :141-142

[8] SECD AND SECF FACILITATE PROTEIN EXPORT IN ESCHERICHIA-COLI [J].

POGLIANO, JA ;

BECKWITH, J .

EMBO JOURNAL, 1994, 13 (03) :554-561

[9] EXPORT OF MALTOSE-BINDING PROTEIN SPECIES WITH ALTERED CHARGE-DISTRIBUTION SURROUNDING THE SIGNAL PEPTIDE HYDROPHOBIC CORE IN ESCHERICHIA-COLI-CELLS HARBORING PRL SUPPRESSOR MUTATIONS [J].

PUZISS, JW ;

STROBEL, SM ;

BASSFORD, PJ .

JOURNAL OF BACTERIOLOGY, 1992, 174 (01) :92-101

[10] DISTINCT MUTATION SITES IN PRLA SUPPRESSOR MUTANT STRAINS OF ESCHERICHIA-COLI RESPOND EITHER TO SUPPRESSION OF SIGNAL PEPTIDE MUTATIONS OR TO BLOCKAGE OF STAPHYLOKINASE PROCESSING [J].

SAKO, T ;

IINO, T .

JOURNAL OF BACTERIOLOGY, 1988, 170 (11) :5389-5391