A thermally baffled device for highly stabilized convective PCR

被引：69

作者：

Chang, Hsiao-Fen Grace ^{[3
]}

Tsai, Yun-Long ^{[3
]}

Tsai, Chuan-Fu ^{[3
]}

Lin, Ching-Ko ^{[3
]}

Lee, Pei-Yu ^{[4
]}

Teng, Ping-Hua ^{[3
]}

Su, Chen ^{[3
]}

Jeng, Chien-Chung ^{[1
,2
]}

机构：

[1] Natl Chung Hsing Univ, Inst Nanosci, Taichung 407, Taiwan

[2] Natl Chung Hsing Univ, Dept Phys, Taichung 407, Taiwan

[3] GeneReach Biotechnol Corp, Dept Res & Dev, Taichung, Taiwan

[4] Cent Taiwan Univ Sci & Technol, Inst Med Biotechnol, Taichung, Taiwan

来源：

BIOTECHNOLOGY JOURNAL | 2012年 / 7卷 / 05期

关键词：

Methods; Rayleigh-Benard convective PCR; Thermally baffled PCR instrument; Yellow head virus; DIAGNOSIS; AMPLIFICATION; PATHOGENS; CELL;

D O I：

10.1002/biot.201100453

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Rayleigh-Benard convective PCR is a simple and effective design for amplification of DNA. Convective PCR is, however, extremely sensitive to environmental temperature fluctuations, especially when using small- diameter test tubes. Therefore, this method is inherently unstable with limited applications. Here, we present a convective PCR device that has been modified by adding thermal baffles. With this thermally baffled device the influence from fluctuations in environmental temperature were significantly reduced, even in a wind tunnel (1 m/s). The thermally baffled PCR instrument described here has the potential to be used as a low-cost, point-of-care device for PCR-based molecular diagnostics in the field.

引用

页码：662 / 666

页数：5

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