ISCOMs vaccine against experimental leishmaniasis

被引：31

作者：

Papadopoulou, G ^{[1
]}

Karagouni, E ^{[1
]}

Dotsika, E ^{[1
]}

机构：

[1] Hellen Pasteur Inst, Athens 11521, Greece

来源：

VACCINE | 1998年 / 16卷 / 9-10期

关键词：

ISCOMs; Leishmania; gp63;

D O I：

10.1016/S0264-410X(97)00308-3

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

The major surface glycoprotein (gp63) of Leishmania major incorporated into the immunostimulating complexes (ISCOMs) was used to prefect Balb/c mice against Experimental infection. Two intraperitoneal vaccinations with low doses of gp63 into ISCOMs (gy63-ISCOMs) induced protective immunity in vaccinated mice as indicated by reduced inflammation and suppressed lesions after experimental challenge. An augmented Igc-specific secretion and a specific switching towards the IgG2a isotype was observed in the serum of vaccinated mice. Gp63-ISCOMs primed spleen cells restimulated in vitro with soluble Leishmania antigen (SLA) or live parasites displayed strong gp63-specific proliferative responses and secreted high levels of interleukin-2, interferon gamma and interleukin-10 but not interleukin-4. No delayed type hypersensitivity response to either SLA or LV39 was detected. These data indicate that gp63-ISCOMs induced a protective immunity in the susceptible Balb/c mice against Leishmania challenge modulating the immune response towards a Th1 rather than Th2 type. (C) 1998 Elsevier Science Ltd. All rights reserved.

引用

页码：885 / 892

页数：8

共 71 条

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