Donor-specific antibody detection: comparison of single antigen assay and Luminex crossmatches

被引：10

作者：

Caro-Oleas, J. L. ^{[1
]}

Gonzalez-Escribano, M. F. ^{[1
]}

Toro-Llamas, S. ^{[1
]}

Acevedo, M. J. ^{[1
]}

Martinez-Bravo, M. J. ^{[1
]}

Aguilera, I. ^{[1
]}

Nunez-Roldan, A. ^{[1
]}

机构：

[1] Univ Seville, Hosp Univ Virgen de Rocio, Serv Inmunol, Edificio Labs,Inst Biomed IBIS, Seville 41013, Spain

来源：

TISSUE ANTIGENS | 2010年 / 76卷 / 05期

关键词：

donor-specific antibodies; HLA antibodies; Luminex crossmatches; sensitivity; single antigen assay; TRANSPLANT PATIENTS; HLA ANTIBODY; BEADS; LYMPHOCYTOTOXICITY; IDENTIFICATION;

D O I：

10.1111/j.1399-0039.2010.01531.x

中图分类号：

Q2 [细胞生物学];

学科分类号：

071013 [干细胞生物学];

摘要：

Luminex bead-based assays are routinely used in the study of anti-human leukocyte antigen (HLA) donor-specific antibodies (DSA). Single antigen (SA) assays use beads coated with recombinant antigens whereas Luminex crossmatch (Xm-DSA) tests consist of beads coated with isolated donor-specific HLA molecules. The aim of this study was to compare these techniques used to detect DSA. A total of 24 sera recognizing different HLA class I (seven anti-HLA-A and seven anti-HLA-B) as well as class II (seven anti-HLA-DR and three anti-HLA DQ) specificities by complement dependent cytotoxicity were included in the study. These sera were used undiluted and in serial dilutions to perform both class I and II SA and Xm-DSA assays. In the case of Xm-DSA the same serum was checked with different lysates. A total of 42 lysates were used to perform a total of 61 crossmatches: 42 to detect anti-class I and 19 to detect anti-class II antibodies. The maximum positive dilution was higher for SA in 76% of the class I and in 90% of the class II crossmatches. Those cases with a higher sensitivity of the Xm-DSA could not be explained by a larger number of antigen targets.

引用

页码：398 / 403

页数：6

共 19 条

[1]

Luminex donor-specific crossmatches [J].