Stable isotope probing - linking microbial identity to function

被引：392

作者：

Dumont, MG ^{[1
]}

Murrell, JC ^{[1
]}

机构：

[1] Univ Warwick, Dept Biol Sci, Coventry CV4 7AL, W Midlands, England

来源：

NATURE REVIEWS MICROBIOLOGY | 2005年 / 3卷 / 06期

基金：

英国自然环境研究理事会;

关键词：

D O I：

10.1038/nrmicro1162

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Stable isotope probing (SIP) is a technique that is used to identify the microorganisms in environmental samples that use a particular growth substrate. The method relies on the incorporation of a substrate that is highly enriched in a stable isotope, such as C-13, and the identification of active microorganisms by the selective recovery and analysis of isotope-enriched cellular components. DNA and rRNA are the most informative taxonomic biomarkers and C-13-labelled molecules can be purified from unlabelled nucleic acid by density-gradient centrifugation. The future holds great promise for SIP, particularly when combined with other emerging technologies such as microarrays and metagenomics.

引用

页码：499 / 504

页数：6

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