Rapid blue-light-mediated induction of protein interactions in living cells

被引：557

作者：

Kennedy, Matthew J. ^{[2
]}

Hughes, Robert M. ^{[1
]}

Peteya, Leslie A. ^{[1
]}

Schwartz, Joel W. ^{[2
]}

Ehlers, Michael D. ^{[2
,3
]}

Tucker, Chandra L. ^{[1
]}

机构：

[1] Duke Univ, Dept Biol, Durham, NC 27706 USA

[2] Duke Univ, Med Ctr, Dept Neurobiol, Durham, NC 27710 USA

[3] Pfizer Global Res & Dev, Neurosci Res Unit, Groton, CT USA

来源：

NATURE METHODS | 2010年 / 7卷 / 12期

基金：

美国国家卫生研究院;

关键词：

ARABIDOPSIS; TRANSCRIPTION; ACTIVATION; LIGANDS; DOMAIN; YEAST;

D O I：

10.1038/NMETH.1524

中图分类号：

Q5 [生物化学];

学科分类号：

070307 [化学生物学];

摘要：

Dimerizers allowing inducible control of protein-protein interactions are powerful tools for manipulating biological processes. Here we describe genetically encoded light-inducible protein-interaction modules based on Arabidopsis thaliana cryptochrome 2 and CIB1 that require no exogenous ligands and dimerize on blue-light exposure with subsecond time resolution and subcellular spatial resolution. We demonstrate the utility of this system by inducing protein translocation, transcription and Cre recombinase-mediated DNA recombination using light.

引用

页码：973 / U48

页数：5

共 18 条

[1]

Action spectrum for cryptochrome-dependent hypocotyl growth inhibition in Arabidopsis [J].

Ahmad, M ;

Grancher, N ;

Heil, M ;

Black, RC ;

Giovani, B ;

Galland, P ;

Lardemer, D .

PLANT PHYSIOLOGY, 2002, 129 (02) :774-785

[2]

The signaling state of Arabidopsis cryptochrome 2 contains flavin semiquinone [J].

Banerjee, Roopa ;

Schleicher, Erik ;

Meier, Stefan ;

Viana, Rafael Munoz ;

Pokorny, Richard ;

Ahmad, Margaret ;

Bittl, Robert ;

Batschauer, Alfred .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2007, 282 (20) :14916-14922

[3]

Unnatural ligands for engineered proteins: New tools for chemical genetics [J].

Bishop, A ;

Buzko, O ;

Heyeck-Dumas, S ;

Jung, I ;

Kraybill, B ;

Liu, Y ;

Shah, K ;

Ulrich, S ;

Witucki, L ;

Yang, F ;

Zhang, C ;

Shokat, KM .

ANNUAL REVIEW OF BIOPHYSICS AND BIOMOLECULAR STRUCTURE, 2000, 29 :577-606

[4]

Cryptochrome blue light photoreceptors are activated through interconversion of flavin redox states [J].

Bouly, Jean-Pierre ;

Schleicher, Erik ;

Dionisio-Sese, Maribel ;

Vandenbussche, Fillip ;

Van Der Straeten, Dominique ;

Bakrim, Nadia ;

Meier, Stefan ;

Batschauer, Alfred ;

Galland, Paul ;

Bittl, Robert ;

Ahmad, Margaret .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2007, 282 (13) :9383-9391

[5]

Biochemical and genetic analysis of the yeast proteome with a movable ORF collection [J].

Gelperin, DM ;

White, MA ;

Wilkinson, ML ;

Kon, Y ;

Kung, LA ;

Wise, KJ ;

Lopez-Hoyo, N ;

Jiang, LX ;

Piccirillo, S ;

Yu, HY ;

Gerstein, M ;

Dumont, ME ;

Phizicky, EM ;

Snyder, M ;

Grayhack, EJ .

GENES & DEVELOPMENT, 2005, 19 (23) :2816-2826

[6]

Preparation of organotypic hippocampal slice cultures for long-term live imaging [J].

Gogolla, Nadine ;

Galimberti, Ivan ;

DePaola, Vincenzo ;

Caroni, Pico .

NATURE PROTOCOLS, 2006, 1 (03) :1165-1171

[7]

Regulation of Cre recombinase by ligand-induced complementation of inactive fragments [J].

Jullien, N ;

Sampieri, F ;

Enjalbert, A ;

Herman, JP .

NUCLEIC ACIDS RESEARCH, 2003, 31 (21) :e131

[8]

Syntaxin-4 Defines a Domain for Activity-Dependent Exocytosis in Dendritic Spines [J].

Kennedy, Matthew J. ;

Davison, Ian G. ;

Robinson, Camenzind G. ;

Ehlers, Michael D. .

CELL, 2010, 141 (03) :524-535

[9]

A novel molecular recognition motif necessary for targeting photoactivated phytochrome signaling to specific basic helix-loop-helix transcription factors [J].

Khanna, R ;

Huq, E ;

Kikis, EA ;

Al-Sady, B ;

Lanzatella, C ;

Quail, PH .

PLANT CELL, 2004, 16 (11) :3033-3044

[10]

Genetically encoded photoswitching of actin assembly through the Cdc42-WASP-Arp2/3 complex pathway [J].

Leung, Daisy W. ;

Otomo, Chinatsu ;

Chory, Joanne ;

Rosen, Michael K. .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2008, 105 (35) :12797-12802

← 1 2 →