DDX3 DEAD-box RNA helicase is required for hepatitis C virus RNA replication

被引：220

作者：

Ariumi, Yasuo ^{[1
]}

Kuroki, Misao ^{[1
]}

Abe, Ken-ichi ^{[1
]}

Dansako, Hiromichi ^{[1
]}

Ikeda, Masanori ^{[1
]}

Wakita, Takaji ^{[2
]}

Kato, Nobuyuki ^{[1
]}

机构：

[1] Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Mol Biol, Okayama 7008558, Japan

[2] Natl Inst Infect Dis, Dept Virol 2, Shinjuku Ku, Tokyo 1628640, Japan

来源：

JOURNAL OF VIROLOGY | 2007年 / 81卷 / 24期

关键词：

D O I：

10.1128/JVI.01517-07

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

DDX3, a DEAD-box RNA helicase, binds to the hepatitis C virus (HCV) core protein. However, the role(s) of DDX3 in HCV replication is still not understood. Here we demonstrate that the accumulation of both genome-length HCV RNA (HCV-O, genotype 1b) and its replicon RNA were significantly suppressed in HuH-7-derived cells expressing short hairpin RNA targeted to DDX3 by lentivirus vector transduction. As well, RNA replication of JFH1 (genotype 2a) and release of the core into the culture supernatants were suppressed in DDX3 knockdown cells after inoculation of the cell culture-generated HCVcc. Thus, DDX3 is required for HCV RNA replication.

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收藏

页码：13922 / 13926

页数：5

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