Induction of an interferon response by RNAi vectors in mammalian cells

被引：777

作者：

Bridge, AJ ^{[1
]}

Pebernard, S ^{[1
]}

Ducraux, A ^{[1
]}

Nicoulaz, AL ^{[1
]}

Iggo, R ^{[1
]}

机构：

[1] Swiss Inst Expt Canc Res, CH-1066 Epalinges, Switzerland

来源：

NATURE GENETICS | 2003年 / 34卷 / 03期

关键词：

D O I：

10.1038/ng1173

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

DNA vectors that express short hairpin RNAs (shRNAs) from RNA polymerase III (Pol III) promoters are a promising new tool to reduce gene expression in mammalian cells. shRNAs are processed to small interfering RNAs (siRNAs) of 21 nucleotides (nt) that guide the cleavage of the cognate mRNA by the RNA-induced silencing complex. Although siRNAs are thought to be too short to induce interferon expression, we report here that a substantial number of shRNA vectors can trigger an interferon response.

引用

页码：263 / 264

页数：2

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