Silver sol immunoagglutination assay for determination of human IgG

被引：11

作者：

Gasparyan, Vardan K. ^{[1
]}

机构：

[1] Natl Acad Sci, Inst Biochem, Yerevan 0014, Armenia

来源：

COLLOIDS AND SURFACES B-BIOINTERFACES | 2010年 / 80卷 / 02期

关键词：

Silver; Nanoparticles; IgG; Immunoagglutination; Antigen detection; HOMOGENEOUS IMMUNOASSAY; GOLD NANOPARTICLES;

D O I：

10.1016/j.colsurfb.2010.06.001

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

Immunoagglutination properties of silver nanoparticles sensitized with antibodies to human IgG for determination of IgG were studied. Effects of various pH on sensitization and consequent immunoagglutination process were investigated. It was shown that the best results were obtained when sensitization and immunoagglutination were conducted at isoelectric point of IgG molecule. It was demonstrated that immunoassay with colloidal silver nanoparticles provides the detection of antigen in wide range of concentrations. (C) 2010 Elsevier B.V. All rights reserved.

引用

页码：180 / 183

页数：4

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