Genetic Characteristics, Coreceptor Usage Potential and Evolution of Nigerian HIV-1 Subtype G and CRF02_AG Isolates

被引:16
作者
Ajoge, Hannah O. [1 ,2 ]
Gordon, Michelle L. [2 ]
de Oliveira, Tulio [3 ]
Green, Taryn N. [2 ]
Ibrahim, Sani [4 ]
Shittu, Oladapo S. [5 ]
Olonitola, Stephen O. [1 ]
Ahmad, Aliyu A. [1 ]
Ndung'u, Thumbi [2 ]
机构
[1] Ahmadu Bello Univ, Dept Microbiol, Zaria, Nigeria
[2] Univ KwaZulu Natal, Nelson R Mandela Sch Med, Doris Duke Med Res Inst, HIV Pathogenesis Programme, Durban, South Africa
[3] Univ KwaZulu Natal, Nelson R Mandela Sch Med, Africa Ctr Hlth & Populat Studies, Durban, South Africa
[4] Ahmadu Bello Univ, Dept Biochem, Zaria, Nigeria
[5] Ahmadu Bello Univ, Ahmadu Bello Univ Teaching Hosp, Dept Gyneacol & Obstet, Zaria, Nigeria
来源
PLOS ONE | 2011年 / 6卷 / 03期
关键词
IMMUNODEFICIENCY-VIRUS TYPE-1; T-CELL RESPONSES; GAG PROTEIN; MOLECULAR EPIDEMIOLOGY; SEQUENCE-ANALYSIS; DRUG-RESISTANCE; VIRAL LOAD; OYO-STATE; DIVERSITY; STRAINS;
D O I
10.1371/journal.pone.0017865
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
HIV-1 CRF02_AG and subtype G (HIV-1G) account for most HIV infections in Nigeria, but their evolutionary trends have not been well documented. To better elucidate the dynamics of the epidemic in Nigeria we characterised the gag and env genes of North-Central Nigerian HIV-1 isolates from pregnant women. Of 28 samples sequenced in both genes, the predominant clades were CRF02_AG (39%) and HIV-1G (32%). Higher predicted proportion of CXCR4-tropic (X4) HIV-1G isolates was noted compared to CRF02_AG (p = 0.007, Fisher's exact test). Phylogenetic and Bayesian analysis conducted on our sequences and all the dated available Nigerian sequences on the Los Alamos data base showed that CRF02_AG and HIV-1G entered into Nigeria through multiple entries, with presence of HIV-1G dating back to early 1980s. This study underlines the genetic complexity of the HIV-1 epidemic in Nigeria, possible subtype-specific differences in co-receptor usage, and the evolutionary trends of the predominant HIV-1 strains in Nigeria, which may have implications for the design of biomedical interventions and better understanding of the epidemic.
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页数:12
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