On the molar mass of κ-carrageenan in the course of conformational transition from the disordered to the fundamental ordered form

被引:43
作者
Bongaerts, K
Reynaers, H
Zanetti, F
Paoletti, S
机构
[1] Univ Trieste, Dept Biochem Biophys & Macromol Chem, I-34127 Trieste, Italy
[2] Katholieke Univ Leuven, Dept Chem, Lab Macromol Struct Chem, B-3001 Heverlee, Belgium
[3] POLY Tech SCrl, I-34012 Trieste, Italy
关键词
D O I
10.1021/ma981203z
中图分类号
O63 [高分子化学(高聚物)];
学科分类号
070305 ; 080501 ; 081704 ;
摘要
Weight-average ((M) over bar(w)) molar mass data were obtained by light scattering on aqueous solutions of a sample of kappa-carrageenan with a relative molar mass of about 157 000. They were compared with literature data of several samples, having (M) over bar(w) values from 35 000 to 720 000, using a variety of methods. Temperature and concentration of supporting 1:1 electrolyte were chosen so as to span across the disorderto-order conformational transition, monitored by the change of optical activity. The present comparative study indicates that the single helix is the fundamental ordered (secondary) conformation of kappa-carrageenan in aqueous solution. This conclusion appears to be universally valid on the basis of a very large number of cases, including polymer samples of different average molar mass, in the presence of different counterions and co-ions, and at different temperatures. The single-helical structure is the conformational "building-block" of a higher ("tertiary") ordered structure that stems from the association of(at least) two of such secondary structures according to a topology that cannot be determined by light-scattering data only.
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页码:675 / 682
页数:8
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