Involvement of nitrosative stress in experimental periodontitis in diabetic rats

被引：40

作者：

Nishikawa, Toru ^{[2
]}

Naruse, Keiko ^{[1
]}

Kobayashi, Yasuko

Miyajima, Shinichi ^{[2
]}

Mizutani, Makoto ^{[3
]}

Kikuchi, Takeshi ^{[2
]}

Soboku, Kyoko ^{[2
]}

Nakamura, Nobuhisa

Sokabe, Asako ^{[2
]}

Tosaki, Takahiro

Hata, Masaki ^{[4
]}

Ohno, Norikazu ^{[3
]}

Noguchi, Toshihide ^{[2
]}

Matsubara, Tatsuaki

机构：

[1] Aichi Gakuin Univ, Sch Dent, Dept Internal Med, Chikusa Ku, Nagoya, Aichi 4648651, Japan

[2] Aichi Gakuin Univ, Sch Dent, Dept Periodontol, Nagoya, Aichi 4648651, Japan

[3] Aichi Gakuin Univ, Sch Dent, Dept Oral Anat, Nagoya, Aichi 4648651, Japan

[4] Aichi Gakuin Univ, Sch Dent, Dept Removable Prosthodont, Nagoya, Aichi 4648651, Japan

来源：

JOURNAL OF CLINICAL PERIODONTOLOGY | 2012年 / 39卷 / 04期

关键词：

Periodontitis; diabetes; inducible nitric oxide synthase; nitrosative stress; nitrotyrosine; NITRIC-OXIDE SYNTHASE; TUMOR-NECROSIS-FACTOR; GLYCATION END-PRODUCTS; SMOOTH-MUSCLE-CELLS; GINGIVAL BLOOD-FLOW; MOLECULAR-MECHANISMS; GENE-EXPRESSION; POLY(ADP-RIBOSE) POLYMERASE; VASCULAR DYSFUNCTION; ENDOTHELIAL-CELLS;

D O I：

10.1111/j.1600-051X.2011.01848.x

中图分类号：

R78 [口腔科学];

学科分类号：

100302 [口腔临床医学];

摘要：

Aim: Periodontal disease is highly prevalent and severe in diabetic patients, and is considered one of the diabetic complications. To elucidate how periodontitis progresses in diabetes, we examined an animal model of periodontitis in diabetic rats. Materials and Methods: Two weeks after the induction of diabetes by streptozotocin, surgical nylon thread was ligated around the cervical portion of the unilateral maxillary second molar to induce periodontitis. Periodontitis was evaluated 2 weeks after the ligation by gingival blood flow, mRNA expressions, Western blot analysis, histological examination and micro CT. Results: Ligation-induced severe periodontitis in the diabetic rats, which was apparently shown by the increase of TNF-a and iNOS mRNA expressions and inflammatory cell infiltration in the gingiva and alveolar bone loss. The number of nitrotyrosine, a footprint of nitrosative stress, -positive cells was significantly higher in the periodontitis of the diabetic rats compared with that in the normal rats. Western blot analysis confirmed that the nitrotyrosine was increased in the periodontitis of the diabetic rats. Conclusions: This is the first study to confirm increased nitrosative stress due to periodontitis in diabetic rats. Nitrosative stress may play a crucial role in the exacerbation of periodontitis in diabetic patients.

引用

页码：342 / 349

页数：8

共 56 条

[1]

GINGIVAL BLOOD-FLOW MEASURED WITH A LASER DOPPLER FLOWMETER [J].