Anticancer activity of hydrophobic peptides from soy proteins

被引：113

作者：

Kim, SE

Kim, HH

Kim, JY

Kang, YI

Woo, HJ

Lee, HJ ^{[1
]}

机构：

[1] Seoul Natl Univ, Coll Agr & Life Sci, Dept Food Sci & Technol, Suwon 441744, South Korea

[2] Seoul Natl Univ, Coll Vet Med, Suwon 441744, South Korea

来源：

BIOFACTORS | 2000年 / 12卷 / 1-4期

关键词：

anticancer; peptide; thermoase hydrolyzates; soy protein;

D O I：

10.1002/biof.5520120124

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

An anticancer peptide from soy protein was purified and isolated. Defatted soy protein was hydrolyzed with thermoase and hydrophobic peptides were extracted with ethanol. The peptide extract was fractionated by XAD-2 hydrophobic, gel filtration chromatography, and different C18 HPLCs. Anticancer activity of each fraction was assayed by measuring in vitro cytotoxicity on P388D1, a mouse monocyte macrophage cell line. IC50 value of a peptide fraction from Sephadex G-25 chromatography was 0.16 mg/ml. This peptide fraction at 1 mg/ml significantly affected cell cycle progression by arresting P388D1 at G2/M phases. Finally purified peptide from analytical C18 HPLC was nonapeptide of which molecular weight was 1157 Da and the sequence was X-Met-Leu-Pro-Ser-Tye-Ser-Pro-Tyr.

引用

页码：151 / 155

页数：5

共 11 条

[1]

Antitumour evaluation of dolastatins 10 and 15 and their measurement in plasma by radioimmunoassay [J].