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Abnormal mitochondrial structure in human unfertilized oocytes and arrested embryos
被引:57
作者:
Au, HK
Yeh, TS
Kao, SH
Tzeng, CR
Hsieh, RH
[1
]
机构:
[1] Taipei Med Univ, Sch Nutr & Hlth Sci, Taipei 110, Taiwan
[2] Taipei Med Univ Hosp, Dept Obstet & Gynecol, Taipei, Taiwan
[3] Taipei Med Univ, Ctr Reprod Med & Sci, Taipei 110, Taiwan
[4] Taipei Med Univ, Grad Inst Cell & Mol Biol, Taipei 110, Taiwan
[5] Taipei Med Univ, Sch Med, Grad Inst Biomed Technol, Taipei 110, Taiwan
来源:
ROLE OF THE MITOCHONDRIA IN HUMAN AGING AND DISEASE: FROM GENES TO CELL SIGNALING
|
2005年
/
1042卷
关键词:
embryo;
mitochondria;
oocyte;
D O I:
10.1196/annals.1338.020
中图分类号:
Q5 [生物化学];
Q7 [分子生物学];
学科分类号:
071010 ;
081704 ;
摘要:
To clarify the relationship between mitochondria and embryo development, We collected human unfertilized oocytes, early embryos, and arrested embryos. Unfertilized oocytes and poor-quality embryos were collected, and the ultrastructure of mitochondria was determined by transmission electron micrography. Four criteria for determining the mitochondrial state were mitochondrial morphology, cristae shape, location, and number of mitochondria. In mature oocytes, mitochondria were rounded with arched cristae and a dense matrix and Were distributed evenly in the ooplasm. In pronuclear zygotes, the size and shape of mitochondria were similar to those in mature oocytes; however, mitochondria appeared to migrate and concentrate around pronuclei. In this study, 67% of examined unfertilized oocytes had fewer mitochondria in the cytoplasm. A decreased number of mitochondria located near the nucleus was also demonstrated in 60% of arrested embryos. Fewer differentiated cristae were determined in all three arrested blastocyst stages of embryos. The relative expressions of oxidative phosphorylation genes in oocytes and embryos were also determined. These data imply that inadequate redistribution of mitochondria, unsuccessful mitochondrial differentiation, or decreased mitochondrial transcription may result in poor oocyte fertilization and compromised embryo development..
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页码:177 / 185
页数:9
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