Abnormal mitochondrial structure in human unfertilized oocytes and arrested embryos

被引:57
作者
Au, HK
Yeh, TS
Kao, SH
Tzeng, CR
Hsieh, RH [1 ]
机构
[1] Taipei Med Univ, Sch Nutr & Hlth Sci, Taipei 110, Taiwan
[2] Taipei Med Univ Hosp, Dept Obstet & Gynecol, Taipei, Taiwan
[3] Taipei Med Univ, Ctr Reprod Med & Sci, Taipei 110, Taiwan
[4] Taipei Med Univ, Grad Inst Cell & Mol Biol, Taipei 110, Taiwan
[5] Taipei Med Univ, Sch Med, Grad Inst Biomed Technol, Taipei 110, Taiwan
来源
ROLE OF THE MITOCHONDRIA IN HUMAN AGING AND DISEASE: FROM GENES TO CELL SIGNALING | 2005年 / 1042卷
关键词
embryo; mitochondria; oocyte;
D O I
10.1196/annals.1338.020
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
To clarify the relationship between mitochondria and embryo development, We collected human unfertilized oocytes, early embryos, and arrested embryos. Unfertilized oocytes and poor-quality embryos were collected, and the ultrastructure of mitochondria was determined by transmission electron micrography. Four criteria for determining the mitochondrial state were mitochondrial morphology, cristae shape, location, and number of mitochondria. In mature oocytes, mitochondria were rounded with arched cristae and a dense matrix and Were distributed evenly in the ooplasm. In pronuclear zygotes, the size and shape of mitochondria were similar to those in mature oocytes; however, mitochondria appeared to migrate and concentrate around pronuclei. In this study, 67% of examined unfertilized oocytes had fewer mitochondria in the cytoplasm. A decreased number of mitochondria located near the nucleus was also demonstrated in 60% of arrested embryos. Fewer differentiated cristae were determined in all three arrested blastocyst stages of embryos. The relative expressions of oxidative phosphorylation genes in oocytes and embryos were also determined. These data imply that inadequate redistribution of mitochondria, unsuccessful mitochondrial differentiation, or decreased mitochondrial transcription may result in poor oocyte fertilization and compromised embryo development..
引用
收藏
页码:177 / 185
页数:9
相关论文
共 29 条
[11]   A novel mutation in the mitochondrial 16S rRNA gene in a patient with MELAS syndrome, diabetes mellitus, hyperthyroidism and cardiomyopathy [J].
Hsieh, RH ;
Li, JY ;
Pang, CY ;
Wei, YH .
JOURNAL OF BIOMEDICAL SCIENCE, 2001, 8 (04) :328-335
[12]   The bottleneck: mitochondrial imperatives in oogenesis and ovarian follicular fate [J].
Jansen, RPS ;
de Boer, K .
MOLECULAR AND CELLULAR ENDOCRINOLOGY, 1998, 145 (1-2) :81-88
[13]   Mitochondrial mutations and male infertility [J].
John, JCS ;
Cooke, ID ;
Barratt, CLR .
NATURE MEDICINE, 1997, 3 (02) :124-125
[14]   Multiple deletions of mitochondrial DNA are associated with the decline of motility and fertility of human spermatozoa [J].
Kao, SH ;
Chao, HT ;
Wei, YH .
MOLECULAR HUMAN REPRODUCTION, 1998, 4 (07) :657-666
[15]   MITOCHONDRIAL DEOXYRIBONUCLEIC-ACID DELETIONS IN OOCYTES AND REPRODUCTIVE AGING IN WOMEN [J].
KEEFE, DL ;
NIVENFAIRCHILD, T ;
POWELL, S ;
BURADAGUNTA, S .
FERTILITY AND STERILITY, 1995, 64 (03) :577-583
[16]   Mitochondrial transcription factor A is necessary for mtDNA maintenance and embryogenesis in mice [J].
Larsson, NG ;
Wang, JM ;
Wilhelmsson, H ;
Oldfors, A ;
Rustin, P ;
Lewandoski, M ;
Barsh, GS ;
Clayton, DA .
NATURE GENETICS, 1998, 18 (03) :231-236
[17]   Mitochondrial role in life and death of the cell [J].
Lee, HC ;
Wei, YH .
JOURNAL OF BIOMEDICAL SCIENCE, 2000, 7 (01) :2-15
[18]   Preparation of tungsten powder by the combustion of CaWO4/Mg [J].
Lee, JH ;
Jung, JC ;
Borovinskaya, IP ;
Vershinnikov, VI ;
Won, CW .
METALS AND MATERIALS INTERNATIONAL, 2000, 6 (01) :73-80
[19]   Mitochondria and neuronal survival [J].
Nicholls, DG ;
Budd, SL .
PHYSIOLOGICAL REVIEWS, 2000, 80 (01) :315-360
[20]   AMOUNTS OF MITOCHONDRIAL-DNA AND ABUNDANCE OF SOME MITOCHONDRIAL GENE TRANSCRIPTS IN EARLY MOUSE EMBRYOS [J].
PIKO, L ;
TAYLOR, KD .
DEVELOPMENTAL BIOLOGY, 1987, 123 (02) :364-374