Infrared MALDI mass spectrometry of large nucleic acids

被引:186
作者
Berkenkamp, S
Kirpekar, F
Hillenkamp, F
机构
[1] Univ Munster, Inst Med Phys & Biophys, D-48149 Munster, Germany
[2] Odense Univ, Dept Mol Biol, DK-5230 Odense M, Denmark
关键词
D O I
10.1126/science.281.5374.260
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Mass spectrometry has become an increasingly important tool of high accuracy, efficiency, and speed for the routine analysis of nucleic acids. To make it useful for large-scale sequencing of genomic material as required for example in genotyping and clinical diagnosis, it is necessary to find approaches that allow the analysis of sequences much larger than the 100 nucleotides currently possible. Matrix-assisted laser desorption/ionization (MALDI) mass spectra of synthetic DNA, restriction enzyme fragments of plasmid DNA, and RNA transcripts up to a size of 2180 nucleotides are reported. The demonstrated mass accuracy of 1 percent or better and the sample requirement of a few femtomoles or Less surpass all currently available techniques for the analysis of large nucleic acids. DNA and RNA can be analyzed with only a limited investment in sample purification.
引用
收藏
页码:260 / 262
页数:3
相关论文
共 16 条
  • [11] Genetic diagnosis by polymerase chain reaction and electrospray ionization mass spectrometry: Detection of five base deletion from blood DNA of a familial adenomatous polyposis patient
    Naito, Y
    Ishikawa, K
    Koga, Y
    Tsuneyoshi, T
    Terunuma, H
    Arakawa, R
    [J]. JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, 1997, 8 (07) : 737 - 742
  • [12] Nordhoff E, 1996, MASS SPECTROM REV, V15, P67, DOI 10.1002/(SICI)1098-2787(1996)15:2<67::AID-MAS1>3.0.CO
  • [13] 2-8
  • [14] ION STABILITY OF NUCLEIC-ACIDS IN INFRARED MATRIX-ASSISTED LASER-DESORPTION IONIZATION MASS-SPECTROMETRY
    NORDHOFF, E
    CRAMER, R
    KARAS, M
    HILLENKAMP, F
    KIRPEKAR, F
    KRISTIANSEN, K
    ROEPSTORFF, P
    [J]. NUCLEIC ACIDS RESEARCH, 1993, 21 (15) : 3347 - 3357
  • [15] NUI S, 1998, J AM SOC MASS SPECTR, V9, P1
  • [16] Analysis of short tandem repeat polymorphisms in human DNA by matrix-assisted laser desorption ionization mass spectrometry
    Ross, PL
    Belgrader, P
    [J]. ANALYTICAL CHEMISTRY, 1997, 69 (19) : 3966 - 3972