Highly sensitive nitrite biosensor based on the electrical wiring of nitrite reductase by [ZnCr-AQS] LDH

被引：84

作者：

Chen, H.

Mousty, C. ^{[1
]}

Cosnier, S.

Silveira, C.

Moura, J. J. G.

Almeida, M. G.

机构：

[1] Univ Grenoble 1, CNRS, UMR 5250, ICMG FR 2607,Dept Chim Mol, F-38041 Grenoble, France

[2] Tongji Univ, Coll Environm Sci & Engn, State Key Lab Pollut Control & Resource Reuse, Shanghai 200092, Peoples R China

[3] Univ Nova Lisboa, Fac Ciencias & Tecnol, Dept Quim, Requimte CQFB, P-1200 Lisbon, Portugal

[4] Escola Super Saude Egas Moniz, P-2829511 Caparica, Portugal

来源：

ELECTROCHEMISTRY COMMUNICATIONS | 2007年 / 9卷 / 09期

关键词：

biosensor; nitrite reductase; layered double hydroxides; anthraquinone sulfonate; nitrite; LAYERED DOUBLE HYDROXIDES; DESULFOVIBRIO-DESULFURICANS ATCC-27774; CLAY-MODIFIED ELECTRODES; METHYL VIOLOGEN; CYTOCHROME; ENZYMES; NITRATE; DESIGN; NRFH;

D O I：

10.1016/j.elecom.2007.05.030

中图分类号：

O646 [电化学、电解、磁化学];

学科分类号：

081704 ;

摘要：

A biosensor for amperometric determination of nitrite was developed using cytochrome c nitrite reductase (ccNiR) from Desulfovibrio desulfuricans immobilized and electrically connected on a glassy carbon electrode by entrapment into redox active [ZnCr-AQS] layered double hydroxide containing anthraquinone-2-sulfonate (AQS). The transduction step corresponded to the electro-enzymatic reduction of nitrite by immobilized AQS molecules at -0.6 V. The biosensor showed a fast response to nitrite (5 s) with a linear range between 0.015 and 2.35 mu M, a sensitivity of 1.8 A M-1 cm(-2) and a detection limit of 4 nM. The apparent Michaelis-Menten constant (K-M(app)) M was 7.5 mu M. (c) 2007 Elsevier B.V. All rights reserved.

引用

页码：2240 / 2245

页数：6

共 28 条

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