学术探索
学术期刊
新闻热点
数据分析
智能评审

Highly sensitive nitrite biosensor based on the electrical wiring of nitrite reductase by [ZnCr-AQS] LDH

被引：84
作者：
Chen, H.
Mousty, C. [1 ]
Cosnier, S.
Silveira, C.
Moura, J. J. G.
Almeida, M. G.
机构：
[1] Univ Grenoble 1, CNRS, UMR 5250, ICMG FR 2607,Dept Chim Mol, F-38041 Grenoble, France
[2] Tongji Univ, Coll Environm Sci & Engn, State Key Lab Pollut Control & Resource Reuse, Shanghai 200092, Peoples R China
[3] Univ Nova Lisboa, Fac Ciencias & Tecnol, Dept Quim, Requimte CQFB, P-1200 Lisbon, Portugal
[4] Escola Super Saude Egas Moniz, P-2829511 Caparica, Portugal
来源：
ELECTROCHEMISTRY COMMUNICATIONS | 2007年 / 9卷 / 09期
关键词：
biosensor; nitrite reductase; layered double hydroxides; anthraquinone sulfonate; nitrite; LAYERED DOUBLE HYDROXIDES; DESULFOVIBRIO-DESULFURICANS ATCC-27774; CLAY-MODIFIED ELECTRODES; METHYL VIOLOGEN; CYTOCHROME; ENZYMES; NITRATE; DESIGN; NRFH;
D O I：
10.1016/j.elecom.2007.05.030
中图分类号：
O646 [电化学、电解、磁化学];
学科分类号：
081704 ;
摘要：
A biosensor for amperometric determination of nitrite was developed using cytochrome c nitrite reductase (ccNiR) from Desulfovibrio desulfuricans immobilized and electrically connected on a glassy carbon electrode by entrapment into redox active [ZnCr-AQS] layered double hydroxide containing anthraquinone-2-sulfonate (AQS). The transduction step corresponded to the electro-enzymatic reduction of nitrite by immobilized AQS molecules at -0.6 V. The biosensor showed a fast response to nitrite (5 s) with a linear range between 0.015 and 2.35 mu M, a sensitivity of 1.8 A M-1 cm(-2) and a detection limit of 4 nM. The apparent Michaelis-Menten constant (K-M(app)) M was 7.5 mu M. (c) 2007 Elsevier B.V. All rights reserved.
引用
收藏
页码：2240 / 2245
页数：6
相关论文
共 28 条
[21]   Application of nitrite reductase from Alcaligenes faecalis S-6 for nitrite measurement [J].
Sasaki, S ;
Karube, I ;
Hirota, N ;
Arikawa, Y ;
Nishiyama, M ;
Kukimoto, M ;
Horinouchi, S ;
Beppu, T .
BIOSENSORS & BIOELECTRONICS, 1998, 13 (01) :1-5
[22]   ELECTROCHEMICAL STUDIES ON NITRITE REDUCTASE TOWARDS A BIOSENSOR [J].
SCHARF, M ;
MORENO, C ;
COSTA, C ;
VANDIJK, C ;
PAYNE, WJ ;
LEGALL, J ;
MOURA, I ;
MOURA, JJG .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1995, 209 (03) :1018-1025
[23]   Layered double hydroxides: An attractive material for electrochemical biosensor design [J].
Shan, D ;
Cosnier, S ;
Mousty, C .
ANALYTICAL CHEMISTRY, 2003, 75 (15) :3872-3879
[24]   HRP/[Zn-Cr-ABTS] redox clay-based biosensor: design and optimization for cyanide detection [J].
Shan, D ;
Cosnier, S ;
Mousty, C .
BIOSENSORS & BIOELECTRONICS, 2004, 20 (02) :390-396
[25]   HRP wiring by redox active layered double hydroxides:: Application to the mediated H2O2 detection [J].
Shan, D ;
Cosnier, S ;
Mousty, C .
ANALYTICAL LETTERS, 2003, 36 (05) :909-922
[26]   ARTIFICIAL ELECTRON-DONORS FOR NITRATE AND NITRITE REDUCTASES USABLE AS MEDIATORS IN APEROMETRIC BIOSENSORS [J].
STREHLITZ, B ;
GRUNDIG, B ;
VORLOP, KD ;
BARTHOLMES, P ;
KOTTE, H ;
STOTTMEISTER, U .
FRESENIUS JOURNAL OF ANALYTICAL CHEMISTRY, 1994, 349 (8-9) :676-678
[27]   A nitrite sensor based on a highly sensitive nitrite reductase mediator-coupled amperometric detection [J].
Strehlitz, B ;
Grundig, B ;
Schumacher, W ;
Kroneck, PMH ;
Vorlop, KD ;
Kotte, H .
ANALYTICAL CHEMISTRY, 1996, 68 (05) :807-816
[28]   A nitrite biosensor based on a maltose binding protein nitrite reductase fusion immobilized on an electropolymerized film of a pyrrole derived bipyridinium [J].
Wu, Q ;
Storrier, GD ;
Pariente, F ;
Wang, Y ;
Shapleigh, JP ;
Abruna, HD .
ANALYTICAL CHEMISTRY, 1997, 69 (23) :4856-4863
123