HRP wiring by redox active layered double hydroxides:: Application to the mediated H2O2 detection

被引：39

作者：

Shan, D ^{[1
]}

Cosnier, S ^{[1
]}

Mousty, C ^{[1
]}

机构：

[1] Univ Grenoble 1, CNRS, FR 2607,Inst Chim Mol Grenoble, Lab Electrochim Organ & Photochim Redox,UMR 5630, F-38041 Grenoble 9, France

来源：

ANALYTICAL LETTERS | 2003年 / 36卷 / 05期

关键词：

layered double hydroxides; anionic clay; amperometric biosensor; horseradish peroxidase; glucose oxidase;

D O I：

10.1081/AL-120019252

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

With the aim of amperometric biosensor development, enzymes were immobilized within layered double hydroxides (LDH), also called anionic clay. Synthetic redox active [Zn-Cr-ABTS] LDH is used as an immobilization matrix for HRP in order to perform the electrical wiring of the enzyme. The electrochemical transduction step corresponds to the reduction at 0.0V of the intercalated ABTS(+), enzymatically formed in the presence of H2O2. The HRP/[Zn-Cr-ABTS] biosensor sensitivity and detection limit are 443mAM(-1) cm(-2) and 10nM, respectively. The co-immobilization of GOx with HPR at [Zn-Cr-ABTS] coating gives rise to a bienzymatic electrode applied to the determination of glucose at 0.0 V.

引用

页码：909 / 922

页数：14

共 30 条

[1] The hydrophilic and lipophilic contribution to total antioxidant activity [J].

Arnao, MB ;

Cano, A ;

Acosta, M .

FOOD CHEMISTRY, 2001, 73 (02) :239-244

[2] Hydrogen peroxide biosensor based on sol-gel-derived glasses doped with Eastman AQ polymer [J].

Chen, X ;

Zhang, JZ ;

Wang, BQ ;

Cheng, GJ ;

Dong, SJ .

ANALYTICA CHIMICA ACTA, 2001, 434 (02) :255-260

[3] Amperometric detection of pyridine nucleotides via immobilized viologen-accepting pyridine nucleotide oxidoreductase or immobilized diaphorase [J].

Cosnier, S ;

LeLous, K .

TALANTA, 1996, 43 (03) :331-337

[4] A new strategy for the construction of amperometric dehydrogenase electrodes based on laponite gel-methylene blue polymer as the host matrix [J].

Cosnier, S ;

LeLous, K .

JOURNAL OF ELECTROANALYTICAL CHEMISTRY, 1996, 406 (1-2) :243-246

[5] Mesoporous TiO2 films:: New catalytic electrode materials for fabricating amperometric biosensors based on oxidases [J].

Cosnier, S ;

Gondran, C ;

Senillou, A ;

Gratzel, M ;

Vlachopoulos, N .

ELECTROANALYSIS, 1997, 9 (18) :1387-1392

[6]

Cosnier S, 2000, ELECTROANAL, V12, P356, DOI 10.1002/(SICI)1521-4109(20000301)12:5<356::AID-ELAN356>3.3.CO

[7]

2-Q

[8] ELECTROCHEMICALLY CONTROLLED-RELEASE OF CHEMICALS FROM REDOX-ACTIVE POLYMER-FILMS [J].

COSNIER, S ;

INNOCENT, C ;

MOUTET, JC ;

TENNAH, F .

JOURNAL OF ELECTROANALYTICAL CHEMISTRY, 1994, 375 (1-2) :233-241

[9] Urea biosensors based on immobilization of urease into two oppositely charged clays (Laponite and Zn-Al layered double hydroxides) [J].

de Melo, JV ;

Cosnier, S ;

Mousty, C ;

Martelet, C ;

Jaffrezic-Renault, N .

ANALYTICAL CHEMISTRY, 2002, 74 (16) :4037-4043

[10]

de Roy A., 1991, Expanded Clays and Other Microporous Solids, P108, DOI [DOI 10.1007/978-1-4684-8866-1_7, 10.1007/978-1-4684-8866-1_7]

← 1 2 3 →