Application of high-precision isotope ratio monitoring mass spectrometry to identify the biosynthetic origins of proteins

被引：6

作者：

Apostol, I

Brooks, PD

Mathews, AJ

机构：

[1] Baxter Hemoglobin Therapeut, Boulder, CO 80301 USA

[2] Univ Calif Berkeley, Berkeley, CA 94720 USA

来源：

PROTEIN SCIENCE | 2001年 / 10卷 / 07期

关键词：

isotope ratio monitoring mass spectrometry; hemoglobin; origin of protein expression;

D O I：

10.1110/ps.90101

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

Isotope ratio monitoring (IRM) mass spectrometry was used to measure the relative abundance of stable isotopes in several samples of adult human hemoglobin expressed in E. coli, yeast, and human blood. The results showed significant differences in the distribution of N-15 and C-13 isotopes among hemoglobin samples produced in these organisms. This indicates that IRM mass spectrometry can be used in forensic protein chemistry to identify the origin of protein expression.

引用

页码：1466 / 1469

页数：4

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