Effect of leukotriene D4 on ciliary activity in human, guinea-pig and rat respiratory mucosa

被引:11
作者
Joki, S
Saano, V
Koskela, T
Toskala, E
Bray, MA
Nuutinen, J
机构
[1] KUOPIO UNIV HOSP,SF-70210 KUOPIO,FINLAND
[2] CIBA GEIGY LTD,DIV PHARMACEUT,RES DEPT,BASEL,SWITZERLAND
来源
PULMONARY PHARMACOLOGY & THERAPEUTICS | 1996年 / 9卷 / 04期
关键词
ciliary activity; guinea-pig; human leukotriene D4; rat;
D O I
10.1006/pulp.1996.0029
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
Leukotriene D4 (LTD4) is a potent bronchoconstrictor and inflammatory mediator in asthma, Data concerning the effects of LTD4 on ciliary function in the respiratory tract are sparse and contradictory, The purpose of the present study was to clarify the effects of LTD4 on mucociliary activity using the tracheal mucosa of two laboratory animal species, guinea-pig and rat, as well as human nasal mucosa, The ciliary beat frequency (CBF) was measured photoelectrically and determined by Fast Fourier Transform computer analysis, Additionally the structure of ciliated epithelia of guinea-pig trachea after LTD4-immersion was investigated using a scanning electron microscope (SEM). In all tissues, LTD4 increased CBF showing a bell-shaped dose-response curve, The maximum effect was 75+/-30% in guinea-pig at 10(-9)mol/l, 119+/-49% in rat at 10(-7)mol/l, and 86+/-28% at 10(-6) mol/l in human tissue, In guinea-pig tracheal mucosa, there was an indication of an increase in the amount of mucus and disorientation of cilia were seen by SEM after immersion in LTD4, These findings suggest that LTD4 stimulates ciliary activity, but impairs the orientation of cilia, (C) 1996 Academic Press Limited.
引用
收藏
页码:231 / 238
页数:8
相关论文
共 36 条
[1]  
BARNES PJ, 1988, PHARMACOL REV, V40, P49
[2]   EFFECT OF LEUKOTRIENE D4 ON NASAL MUCOSAL BLOOD-FLOW, NASAL AIRWAY-RESISTANCE AND NASAL SECRETION IN HUMANS [J].
BISGAARD, H ;
OLSSON, P ;
BENDE, M .
CLINICAL ALLERGY, 1986, 16 (04) :289-297
[3]   SRS-A LEUKOTRIENES DECREASE THE ACTIVITY OF HUMAN RESPIRATORY CILIA [J].
BISGAARD, H ;
PEDERSEN, M .
CLINICAL ALLERGY, 1987, 17 (02) :95-103
[4]   THE CALDWELL-LUC PROCEDURE IN 1991 [J].
BLITZER, A ;
LAWSON, W .
OTOLARYNGOLOGY-HEAD AND NECK SURGERY, 1991, 105 (05) :717-722
[5]   THE INFLUENCE OF LEUKOTRIENES AND PLATELET ACTIVATING FACTOR ON MUCOCILIARY ACTIVITY IN THE RABBIT MAXILLARY SINUS [J].
DOLATA, J ;
LINDBERG, S ;
MERCKE, U .
ACTA OTO-LARYNGOLOGICA, 1990, 109 (1-2) :149-154
[6]   SPUTUM AND CILIARY INHIBITION IN ASTHMA [J].
DULFANO, MJ ;
LUK, CK .
THORAX, 1982, 37 (09) :646-651
[7]   EFFECT OF KLEBSIELLA-OZAENAE ON CILIARY ACTIVITY INVITRO - IMPLICATIONS IN THE PATHOGENESIS OF ATROPHIC RHINITIS [J].
FERGUSON, JL ;
MCCAFFREY, TV ;
KERN, EB ;
MARTIN, WJ .
OTOLARYNGOLOGY-HEAD AND NECK SURGERY, 1990, 102 (03) :207-211
[8]   INHIBITION OF MUCOCILIARY CLEARANCE OF THE EUSTACHIAN-TUBE BY LEUKOTRIENES C4 AND D4 [J].
GANBO, T ;
HISAMATSU, K ;
SHIMOMURA, S ;
NAKAJIMA, T ;
INOUE, H ;
MURAKAMI, Y .
ANNALS OF OTOLOGY RHINOLOGY AND LARYNGOLOGY, 1995, 104 (03) :231-236
[9]  
HOLROYDE MC, 1981, LANCET, V2, P17
[10]   CILIARY BEAT FREQUENCY AT 6 LEVELS OF THE RESPIRATORY-TRACT IN COW, DOG, GUINEA-PIG, PIG, RABBIT AND RAT [J].
JOKI, S ;
SAANO, V .
CLINICAL AND EXPERIMENTAL PHARMACOLOGY AND PHYSIOLOGY, 1994, 21 (05) :427-434