Genome-wide identification of Dicer-like, Argonaute and RNA-dependent RNA polymerase gene families and their expression analyses in response to viral infection and abiotic stresses in Solanum lycopersicum

被引：186

作者：

Bai, Miao ^{[2
]}

Yang, Guo-Shun ^{[2
]}

Chen, Wen-Ting ^{[2
]}

Mao, Zhen-Chuan ^{[1
]}

Kang, Hou-Xiang ^{[1
]}

Chen, Guo-Hua ^{[1
]}

Yang, Yu-Hong ^{[1
]}

Xie, Bing-Yan ^{[1
]}

机构：

[1] Chinese Acad Agr Sci, Inst Vegetables & Flowers, Beijing 100081, Peoples R China

[2] Hunan Agr Univ, Key Lab Crop Germplasm Innovat & Utilizat Hunan P, Changsha 410128, Hunan, Peoples R China

来源：

GENE | 2012年 / 501卷 / 01期

基金：

中国国家自然科学基金;

关键词：

RNA silencing; MicroRNA; Phylogenetic analysis; Tomato yellow leaf curl virus;

D O I：

10.1016/j.gene.2012.02.009

中图分类号：

Q3 [遗传学];

学科分类号：

071007 [遗传学];

摘要：

Dicer, Argonaute and RNA-dependent RNA polymerase form the core components to trigger RNA silencing. Although tomato (Solanum lycopersicum) is a dicotyledon model plant, no systematic analysis and expression profiling of these genes in tomato has been undertaken previously. In this study, seven Dicer-like (SIDCLs),15 Argonaute (SIAGOs) and six RNA-dependent RNA polymerase (SIRDRs) genes were identified in tomato. These genes were categorized into four subgroups based on phylogenetic analyses. Comprehensive analyses of gene structure, genomic localization and similarity among these genes were performed. Their expression patterns were investigated by means of expression models in different tissues and organs using online data and semi-quantitative RT-PCR. Many of the candidate genes were up-regulated in response to Tomato yellow leaf curl virus infection and abiotic stresses. The expression models of tandem gene duplications among SIDCL2s indicated the DCL2 family plays an important role in the evolution of tomato. (C) 2012 Elsevier B.V. All rights reserved.

引用

页码：52 / 62

页数：11

共 52 条

[1]

Molecular characterization of geminivirus-derived small RNAs in different plant species [J].