Cloning and characterization of human estrogen receptor β isoforms

被引:445
作者
Moore, JT
McKee, DD
Slentz-Kesler, K
Moore, LB
Jones, SA
Horne, EL
Su, JL
Kliewer, SA
Lehmann, JM
Willson, TM
机构
[1] Glaxo Wellcome Res & Dev, Dept Mol Sci, Res Triangle Pk, NC 27709 USA
[2] Glaxo Wellcome Res & Dev, Dept Mol Endocrinol, Res Triangle Pk, NC 27709 USA
[3] Glaxo Wellcome Res & Dev, Dept Med Chem, Res Triangle Pk, NC 27709 USA
关键词
D O I
10.1006/bbrc.1998.8738
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Multiple transcripts which arise from the human estrogen receptor beta (ER beta) gene have been characterized. Three full length isoforms of the hER beta gene, designated hER beta 1-3, were identified in a testis cDNA library. An additional two isoforms, designated hER beta 4 and hER beta 5, were identified by PCR amplification from testis cDNA and from the MDA-MB 435 cell line. hER beta 1 corresponds to the previously described hER beta. All five isoforms diverge at a common position within the predicted helix 10 of the ligand binding domain of hER beta, with nucleotide sequences consistent with differential exon usage. The hER beta isoform mRNAs displayed a differential pattern of expression in human tissues and in tumor cell lines when analyzed by RT-PCR. Further characterization of the three full length isoforms, hER beta 1-3, by in vitro band shift studies indicated that the isoforms were able to form DNA-binding homodimers and heterodimers with each other and with the ER alpha subtype. (C) 1998 Academic Press.
引用
收藏
页码:75 / 78
页数:4
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