The rapid determination of artemisinin by post-column derivatization high-performance liquid chromatography using matrix solid-phase dispersion method

被引：24

作者：

Liu, Hongcheng ^{[1
]}

Li, Qiwan ^{[1
]}

Li, Shaoping ^{[2
]}

Zou, Yanhong ^{[1
]}

Gu, Anyun ^{[2
]}

机构：

[1] Minist Agr, Yunnan Acad Agr Sci, Qual Stand & Testing Technol Inst, Kunming Supervis & Testing Ctr Farm Prod Qual, Kunming 650223, Peoples R China

[2] Med Plants Res Inst, Kunming 650223, Peoples R China

来源：

JOURNAL OF CHROMATOGRAPHIC SCIENCE | 2008年 / 46卷 / 02期

关键词：

D O I：

10.1093/chromsci/46.2.122

中图分类号：

Q5 [生物化学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

Artemisinin (an antimalaric compound) is isolated as the active compound of the medicinal plant Artemisia annua L. A simple, rapid, and high-efficient method of extraction is developed, in which it is extracted by matrix solid-phase dispersion (MSPD) and directly analyzed by post-column derivatization high-performance liquid chromatography (HPLC). The quantitation results from the MSPD method are compared with two conventional liquid solvent extraction processes, Soxhlet and ultrasonic wave by F-test, and the result indicates no significant difference. The production rate of Artemisinin during wild plant growth (tested over two years, acquired from Yunnan, provincial Yuan Yan country) is determined. The recovery range of determination is 88.1-91.2%. The relative standard deviation (RSD) is 4.55-6.43%. The limits of detection are 0.1 μg/mL, and the limits of quantitation are 0.5 μg/mL.

引用

页码：122 / 126

页数：5

共 21 条

[1]

Determination of artemisinin in human serum by high-performance liquid chromatography with on-line UV irradiation and peroxyoxalate chemiluminescence detection [J].