Genetic diversity of nodulating and non-nodulating rhizobia associated with wild soybean (Glycine soja Sieb. & Zucc.) in different ecoregions of China

被引：43

作者：

Wu, Li Juan ^{[1
]}

Wang, Hai Qing ^{[1
]}

Wang, En Tao ^{[2
]}

Chen, Wen Xin ^{[1
]}

Tian, Chang Fu ^{[1
]}

机构：

[1] China Agr Univ, Coll Biol Sci, Dept Microbiol & Immunol, State Key Lab Agro Biotechnol, Beijing 100193, Peoples R China

[2] Inst Politecn Nacl, Escuela Nacl Ciencias Biol, Dept Microbiol, Mexico City, DF, Mexico

来源：

FEMS MICROBIOLOGY ECOLOGY | 2011年 / 76卷 / 03期

基金：

中国国家自然科学基金;

关键词：

geographic distribution; Glycine soja; phylogeny; symbiosis; MULTILOCUS SEQUENCE-ANALYSIS; SP-NOV; BRADYRHIZOBIUM-JAPONICUM; EVOLUTIONARY GENETICS; MOLECULAR DIVERSITY; ROOT-NODULES; VICIA-FABA; GENUS; POPULATION; BACTERIA;

D O I：

10.1111/j.1574-6941.2011.01064.x

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A total of 99 bacterial isolates that originated from root nodules of Glycine soja were characterized with restriction analyses of amplified 16S ribosomal DNA and 16S-23S rDNA intergenic spacers (ITS), and sequence analyses of 16S rRNA, rpoB, atpD, recA and nodC genes. When tested for nodulation of G. soja, 72 of the isolates were effective symbionts, and these belonged to five species: Bradyrhizobium japonicum, Bradyrhizobium elkanii, Bradyrhizobium yuanmingense, Bradyrhizobium liaoningense and Sinorhizobium fredii. All of these, except some B. yuanmingense strains, also formed effective nodules on the domesticated soybean Glycine max. The remaining 27 isolates did not nodulate either host, but were identified as Rhizobium. Phylogeny nodC in the G. soja symbionts suggested that this symbiosis gene was mainly maintained by vertical gene transfer. Different nodC sublineages and rrs-ITS clusters reflected the geographic origins of isolates in this study.

引用

页码：439 / 450

页数：12

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