LOXL1 deficiency negatively impacts the biomechanical properties of the mouse vagina and supportive tissues

被引:43
作者
Alperin, Marianna [1 ,2 ]
Debes, Kristen [2 ]
Abramowitch, Steven [3 ]
Meyn, Leslie
Moalli, Pamela A. [1 ,2 ]
机构
[1] Univ Pittsburgh, Magee Womens Hosp, Dept Obstet Gynecol & Reprod Sci, Div Urogynecol, Pittsburgh, PA 15213 USA
[2] Univ Pittsburgh, Magee Womens Res Inst, Pittsburgh, PA 15213 USA
[3] Univ Pittsburgh, Musculoskeletal Res Ctr, Dept Bioengn, Pittsburgh, PA 15213 USA
关键词
pelvic floor; elastin; prolapse; LOXL1;
D O I
10.1007/s00192-008-0561-7
中图分类号
R71 [妇产科学];
学科分类号
100211 [妇产科学];
摘要
Mice deficient in lysyl oxidase-like1 protein (LOXL1(-/-)) develop pelvic organ prolapse (POP). We sought to determine the impact of LOXL1(-/-) on the biomechanical properties of the vagina and its supportive tissues tested as a complex. Tissues of nulliparous LOXL1(-/-) and age-matched wild type (WT) mice were tested to failure to obtain load-distension curves. Data were compared utilizing one-way analysis of variance and appropriate post hoc tests. The groups demonstrated different biomechanical behavior, with LOXL1(-/-) animals displaying a 31% decrease in ultimate load at failure (p=0.001). Experimental disruption of specific levels of support in WT mice failed to generate load-distension curves similar to the LOXL1(-/-) mice indicating a global instead of a site-specific tissue defect. The decrease in the ultimate load at failure in the LOXL1(-/-) mice suggests mechanically weaker tissues. LOXL1 mutation results in a global defect in connective tissues and correlates with altered biomechanical behavior of the vagina and supportive tissues.
引用
收藏
页码:977 / 986
页数:10
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