Identification of D-aspartate in rat pheochromocytoma PC12 cells

被引：10

作者：

Moriyama, Y ^{[1
]}

Yamada, H ^{[1
]}

Hayashi, M ^{[1
]}

Oda, T ^{[1
]}

Yamaguchi, A ^{[1
]}

机构：

[1] Osaka Univ, Inst Sci & Ind Res, Dept Cell Membrane Biol, Ibaraki, Osaka 567, Japan

来源：

NEUROSCIENCE LETTERS | 1998年 / 248卷 / 01期

关键词：

D-aspartate; PC12; cells; de novo biosynthesis; pineal gland; endocrine cell; mammalian cell; high performance liquid chromatography; immunohistochemistry;

D O I：

10.1016/S0304-3940(98)00308-5

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

D-Aspartate is now known to be present in mammalian neuronal and endocrine cells in vivo, and may play some role(s) in neurocrine and endocrine functions. However, origin of D-aspartate is unknown. Here, we report that free D-aspartate (108 pmoles/3 x 10(7) cells) is present in the cultured PC12 cells, a rat pheochromocytoma cell line, as determined with immunohistochemical techniques as well as high performance liquid chromatography (HPLC) on a Pirkle-type chiral column. The amount of D-aspartate does not change with the passage. The culture medium does not contain D-aspartate. These results strongly suggest the presence of a de novo biosynthetic pathway for D-aspartate in the endocrine cells. (C) 1998 Elsevier Science Ireland Ltd.

引用

页码：57 / 60

页数：4