Fluorogenic DNAzyme Probes as Bacterial Indicators

被引：184

作者：

Ali, M. Monsur ^{[1
,2
]}

Aguirre, Sergio D. ^{[1
,2
]}

Lazim, Hadeer ^{[1
,2
]}

Li, Yingfu ^{[1
,2
]}

机构：

[1] McMaster Univ, Dept Biochem & Biomed Sci, Hamilton, ON L8N 3Z5, Canada

[2] McMaster Univ, Dept Chem & Chem Biol, Hamilton, ON L8N 3Z5, Canada

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2011年 / 50卷 / 16期

基金：

加拿大自然科学与工程研究理事会;

关键词：

bacterial detection; biosensors; DNA cleavage; DNAzymes; fluorescence; RNA-CLEAVING DEOXYRIBOZYME; SIGNALING DNA ENZYMES; PATHOGEN DETECTION; CATALYTIC DNA; APTAMERS; PERSPECTIVE; EVOLUTION; SELECTION; DESIGN; PH6DZ1;

D O I：

10.1002/anie.201100477

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

(Figure Presented) Lighting up bacteria: An RNA-cleaving fluorescent DNAzyme (RFD) can produce a fluorescent signal in the crude extracellular mixture generated by live bacterial cells (see picture). These DNAzymes cleave a lone RNA linkage (R) embedded in a DNA chain and flanked by nucleotides labeled with a fluorophore (F) and a quencher (Q). © 2011 Wiley-VCH Verlag GmbH & Co. KGaA.

引用

页码：3751 / 3754

页数：4

共 43 条

[1] Colorimetric Sensing by Using Allosteric-DNAzyme-Coupled Rolling Circle Amplification and a Peptide Nucleic Acid-Organic Dye Probe [J].