Colorimetric Sensing by Using Allosteric-DNAzyme-Coupled Rolling Circle Amplification and a Peptide Nucleic Acid-Organic Dye Probe

被引：122

作者：

Ali, M. Monsur

Li, Yingfu ^{[1
]}

机构：

[1] McMaster Univ, Dept Biochem & Biomed Sci, Hamilton, ON L8N 3Z5, Canada

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2009年 / 48卷 / 19期

关键词：

biosensors; colorimetry; DNAzymes; peptide nucleic acids; rolling circle amplification; IN-VITRO SELECTION; PNA-DNA DUPLEXES; SECONDARY STRUCTURE; PROTEIN-DETECTION; CYANINE DYE; APTAMERS; DEOXYRIBOZYME; OLIGONUCLEOTIDES; MOLECULES; ENZYMES;

D O I：

10.1002/anie.200805966

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Target detection by the naked eye: The action of an RNA-cleaving allosteric DNAzyme in response to ligand binding was coupled to a rolling circle amplification process to generate long single-stranded DNA molecules for colorimetric sensing (see scheme). Upon hybridization of the resulting DNA with a complementary PNA sequence in the presence of a duplex-binding dye, the color of the dye changed from blue to purple. © 2009 Wiley-VCH Verlag GmbH & Co. KGaA.

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页码：3512 / 3515

页数：4

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