Determination of sulfamethazine and trimethoprim in liquid feed premixes by HPLC and diode array detection, with an analysis of the uncertainty of the results

被引：24

作者：

Cancho-Grande, B ^{[1
]}

García-Falcón, MS ^{[1
]}

Rodríguez-Comesaña, M ^{[1
]}

Simal-Gándara, J ^{[1
]}

机构：

[1] Univ Vigo, Fac Food Sci & Technol, Analyt & Food Chem Dept, Nutr & Bromatol Grp, Orense 32004, Spain

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2001年 / 49卷 / 07期

关键词：

antibiotics; diode array; feed premix; liquid chromatography; sulfamethazine; trimethoprim; uncertainty;

D O I：

10.1021/jf010071x

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

Sulfamethazine (SMZ) and trimethoprim (TMP) are antibacterials used in veterinary practice. This paper describes a method for their determination in veterinary liquid feed premixes that is based on liquid chromatography with diode array detection. Gradient elution with methanol and ammonium acetate achieved excellent separation of the two analytes within 15 min without any interference from the matrix. Absorbance of the column effluent was monitored at 264 nm for SMZ and at 230 nm for TMP. Detailed analyses of the uncertainties of determinations afford estimated expanded uncertainties of, respectively, 0.2 and 0.1 w/v % for typical SMZ and TMP concentrations of 10.7 and 2.1 w/v %, respectively. At the lower end of the calibrated range of the method, the dominant source of uncertainty is the preparation of standards and the construction of the calibration line.

引用

页码：3145 / 3150

页数：6

共 14 条

[11] CONCURRENT ANALYSIS OF METHOTREXATE, TRIMETHOPRIM, SULFAMETHOXAZOLE AND THEIR MAJOR METABOLITES IN PLASMA BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY [J].