Retinal transplantation of neural progenitor cells derived from the brain of GFP transgenic mice

被引：41

作者：

Mizumoto, H

Mizumoto, K

Shatos, MA

Klassen, H

Young, MJ

机构：

[1] Harvard Univ, Sch Med, Schepens Eye Res Inst, Dept Ophthalmol, Boston, MA 02114 USA

[2] Childrens Hosp Orange Cty, Stem Cell Res, Orange, CA 92868 USA

来源：

VISION RESEARCH | 2003年 / 43卷 / 16期

关键词：

neural progenitor cells; green fluorescent protein; retinal transplantation; cell differentiation; GFP transgenic mice;

D O I：

10.1016/S0042-6989(03)00235-9

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Neural progenitor cells isolated from the brains of neonatal GFP transgenic mice were grafted to the retina of RCS rats and rds and B6 mice. Expression of GFP and differentiation markers was evaluated at 1-4 weeks post-transplantation. Grafted cells maintained transgene expression throughout the 4-week period. At 1 week there was widespread migration of GFP + cells within the host retina and at 2 weeks evidence of neuronal differentiation (as shown by both marker expression and cell morphology), although integration at 4 weeks was limited to syngeneic recipients. Because brain-derived neural progenitor cells exhibit both neuronal and astrocytic differentiation in diseased and normal host retina, these cells provide a useful tool for studies of retinal regeneration. (C) 2003 Elsevier Science Ltd. All rights reserved.

引用

页码：1699 / 1708

页数：10

共 32 条

[31] Neuronal differentiation and morphological integration of hippocampal progenitor cells transplanted to the retina of immature mature dystrophic rats [J].