Quantification of urinary PGEm, 6-keto PGF1α and 2,3-dinor-6-keto PGF1α by UFLC-MS/MS before and after exercise

被引：14

作者：

Blatnik, Matthew ^{[1
]}

Steenwyk, Rick C. ^{[1
]}

机构：

[1] Pfizer Global Res & Dev, Groton Labs, Dept Pharmacokinet Dynam & Metab, Groton, CT 06340 USA

来源：

PROSTAGLANDINS & OTHER LIPID MEDIATORS | 2010年 / 93卷 / 1-2期

关键词：

2,3-Dinor-6-keto PGF(1 alpha); 6-Keto PEG(1 alpha); Ercosanoids; ESI; Electrospray ionization; Exercise; Inflammation; MS/MS; Tandem mass spectrometry; PGEm; Prostanoids; UFLC; Ultra-flow liquid chromatography; OXIDATIVE STRESS; PROSTAGLANDIN E-2; PHYSICAL-ACTIVITY; IN-VIVO; HUMANS; PGE(2); ASSAY;

D O I：

10.1016/j.prostaglandins.2010.04.002

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Eicosanoids play an important role in the evaluation of pro-inflammatory responses and in the safety and toxicity of novel therapeutic agents. This work describes a high-throughput UFLCMS/MS method for the analysis of three urinary prostanoid biomarkers of pro-inflammatory responses, tetranor PGEm, 6-keto PGF(1 alpha) and 2,3-chnor-6-keto PFG(1 alpha) Nine male volunteers of various age and fitness level participated in this study Six provided pre- and post-exercise samples and three provided intraday samples. Tetranor PGEm and 6-keto PGF(1 alpha) increased significantly in patients after exercise (p < 0 017 and p < 0 029). In individual patient sets, tetranor PC,Em levels increased from 1 5- to 6-fold pre- vs post-exercise, levels of 6-keto PGF(1 alpha) increased more dramatically from 2- to 55-fold pre- vs post-exercise The prostanoid 2,3-dinor-6-keto PGF(1 alpha), remained unchanged post-exercise. Data was normalized to urinary creatinine concentration, which increased approximately 40% post-exercise. (C) 2010 Elsevier Inc All rights reserved

引用

页码：8 / 13

页数：6

共 29 条

[1] Urinary creatinine concentrations in the US population: Implications for urinary biologic monitoring measurements [J].

Barr, DB ;

Wilder, LC ;

Caudill, SP ;

Gonzalez, AJ ;

Needham, LL ;

Pirkle, JL .

ENVIRONMENTAL HEALTH PERSPECTIVES, 2005, 113 (02) :192-200

[2] PROSTAGLANDINS [J].

BERGSTRO.S ;

SAMUELSS.B .

ANNUAL REVIEW OF BIOCHEMISTRY, 1965, 34 :101-&

[3]

BERGSTROM S, 1962, J BIOL CHEM, V237, P3005

[4] MEASUREMENT OF RENAL AND NONRENAL EICOSANOID SYNTHESIS [J].

CATELLA, F ;

NOWAK, J ;

FITZGERALD, GA .

AMERICAN JOURNAL OF MEDICINE, 1986, 81 (2B) :23-29

[5] Simultaneous quantification of 8-iso-prostaglandin-F2α and 11-dehydro thromboxane B2 in human urine by liquid chromatography-tandem mass spectrometry [J].

Cavalca, Viviana ;

Minardi, Fabiana ;

Scurati, Samuele ;

Guidugli, Federico ;

Squellerio, Isabella ;

Veglia, Fabrizio ;

Dainese, Luca ;

Guarino, Anna ;

Tremoli, Elena ;

Caruso, Donatella .

ANALYTICAL BIOCHEMISTRY, 2010, 397 (02) :168-174

[6] Development of enzyme-linked immunosorbent assay for 8-iso-prostaglandin F2α, a biomarker of oxidative stress in vivo, and its application to the quantification in aged rats [J].

Chu, Xiaoqing ;

Ageishi, Yuji ;

Nishimura, Kohji ;

Jisaka, Mitsuo ;

Nagaya, Tsutomu ;

Shono, Fumiaki ;

Yokota, Kazushige .

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, 2009, 50 (05) :911-916

[7] BIOLOGICAL ACTIVITY OF FREE RADICALS [J].

COMMONER, B ;

HEISE, JJ ;

LIPPINCOTT, BB ;

NORBERG, RE ;

PASSONNEAU, JV ;

TOWNSEND, J .

SCIENCE, 1957, 126 (3263) :57-63

[8] Oxidative stress in marathon runners:: interest of antioxidant supplementation [J].

Gomez-Cabrera, Mari-Carmen ;

Martinez, Agustin ;

Santangelo, Gustavo ;

Pallardo, Federico V. ;

Sastre, Juan ;

Vina, Jose .

BRITISH JOURNAL OF NUTRITION, 2006, 96 :S31-S33

[9] Decreasing xanthine oxidase-mediated oxidative stress prevents useful cellular adaptations to exercise in rats [J].

Gomez-Cabrera, MC ;

Borrás, C ;

Pallardó, FV ;

Sastre, J ;

Ji, LL ;

Viña, J .

JOURNAL OF PHYSIOLOGY-LONDON, 2005, 567 (01) :113-120

[10] SUPEROXIDE, IRON, VASCULAR ENDOTHELIUM AND REPERFUSION INJURY [J].

HALLIWELL, B .

FREE RADICAL RESEARCH COMMUNICATIONS, 1989, 5 (06) :315-318

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