Quantification of urinary PGEm, 6-keto PGF1α and 2,3-dinor-6-keto PGF1α by UFLC-MS/MS before and after exercise

被引：14

作者：

Blatnik, Matthew ^{[1
]}

Steenwyk, Rick C. ^{[1
]}

机构：

[1] Pfizer Global Res & Dev, Groton Labs, Dept Pharmacokinet Dynam & Metab, Groton, CT 06340 USA

来源：

PROSTAGLANDINS & OTHER LIPID MEDIATORS | 2010年 / 93卷 / 1-2期

关键词：

2,3-Dinor-6-keto PGF(1 alpha); 6-Keto PEG(1 alpha); Ercosanoids; ESI; Electrospray ionization; Exercise; Inflammation; MS/MS; Tandem mass spectrometry; PGEm; Prostanoids; UFLC; Ultra-flow liquid chromatography; OXIDATIVE STRESS; PROSTAGLANDIN E-2; PHYSICAL-ACTIVITY; IN-VIVO; HUMANS; PGE(2); ASSAY;

D O I：

10.1016/j.prostaglandins.2010.04.002

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Eicosanoids play an important role in the evaluation of pro-inflammatory responses and in the safety and toxicity of novel therapeutic agents. This work describes a high-throughput UFLCMS/MS method for the analysis of three urinary prostanoid biomarkers of pro-inflammatory responses, tetranor PGEm, 6-keto PGF(1 alpha) and 2,3-chnor-6-keto PFG(1 alpha) Nine male volunteers of various age and fitness level participated in this study Six provided pre- and post-exercise samples and three provided intraday samples. Tetranor PGEm and 6-keto PGF(1 alpha) increased significantly in patients after exercise (p < 0 017 and p < 0 029). In individual patient sets, tetranor PC,Em levels increased from 1 5- to 6-fold pre- vs post-exercise, levels of 6-keto PGF(1 alpha) increased more dramatically from 2- to 55-fold pre- vs post-exercise The prostanoid 2,3-dinor-6-keto PGF(1 alpha), remained unchanged post-exercise. Data was normalized to urinary creatinine concentration, which increased approximately 40% post-exercise. (C) 2010 Elsevier Inc All rights reserved

引用

页码：8 / 13

页数：6

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