Molecular cloning of the cDNA and chromosome localization of the gene for human ubiquitin-conjugating enzyme 9

被引：71

作者：

Wang, ZY

Qiu, QQ

Seufert, W

Taguchi, T

Testa, JR

Whitmore, A

Callen, DF

Welsh, D

Shenk, T

Deuel, TF

机构：

[1] BETH ISRAEL HOSP,DEPT MED,BOSTON,MA 02215

[2] HARVARD UNIV,SCH MED,BOSTON,MA 02215

[3] PRINCETON UNIV,DEPT MOL BIOL,PRINCETON,NJ 08560

[4] UNIV MUNICH,INST GENET & MIKROBIOL,D-80638 MUNICH,GERMANY

[5] FOX CHASE CANC CTR,DEPT MED ONCOL,PHILADELPHIA,PA 19111

[6] WOMENS & CHILDRENS HOSP,DEPT CYTOGENET & MOL GENET,ADELAIDE,SA 5006,AUSTRALIA

来源：

JOURNAL OF BIOLOGICAL CHEMISTRY | 1996年 / 271卷 / 40期

关键词：

D O I：

10.1074/jbc.271.40.24811

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We report a novel human gene whose product specifically associates with the negative regulatory domain of the Wilms' tumor gene product (WT1) in a yeast two-hybrid screen and with WT1 in immunoprecipitation and glutathione S-transferase (GST) capture assays. The gene encodes a 17-kDa protein that has 56% amino acid sequence identity with yeast ubiquitin-conjugating enzyme (yUBC) 9, a protein required for cell cycle progression in yeast, and significant identity with other subfamilies of ubiquitin-conjugating enzymes. The human gene fully complements yeast that have a temperature-sensitive yUBC9 gene mutation to fully restore normal growth, indicating that we have cloned a functionally conserved human (h) homolog of yUBC9. Transcripts of hUBC9 of 4.4 kilobases (kb), 2.8 kb, and 1.3 kb were found in all human tissues tested. A single copy of the hUBC9 gene was found and localized to human chromosome 16p13.3. We conclude that hUBC9 retains striking structural and functional conservation with yUBC9 and suggest a possible link of the ubiquitin/proteosome proteolytic pathway and the WT1 transcriptional repressor system.

引用

页码：24811 / 24816

页数：6

共 28 条

[11] GUARENTE L, 1983, METHOD ENZYMOL, V101, P181
[12] HARRINGTON MA, 1993, J BIOL CHEM, V268, P21271
[13] THE UBIQUITIN SYSTEM FOR PROTEIN-DEGRADATION
HERSHKO, A
CIECHANOVER, A
[J]. ANNUAL REVIEW OF BIOCHEMISTRY, 1992, 61 : 761 - 807
[14] THE SHORT-LIVED MAT-ALPHA-2 TRANSCRIPTIONAL REGULATOR IS UBIQUITINATED INVIVO
HOCHSTRASSER, M
ELLISON, MJ
CHAU, V
VARSHAVSKY, A
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (11) : 4606 - 4610
[15] MULTIPLE PROTEOLYTIC SYSTEMS, INCLUDING THE PROTEASOME, CONTRIBUTE TO CFTR PROCESSING
JENSEN, TJ
LOO, MA
PIND, S
WILLIAMS, DB
GOLDBERG, AL
RIORDAN, JR
[J]. CELL, 1995, 83 (01) : 129 - 135
[16] THE UBIQUITIN-CONJUGATION SYSTEM
JENTSCH, S
[J]. ANNUAL REVIEW OF GENETICS, 1992, 26 : 179 - 207
[17] G(1) PHASE ARREST INDUCED BY WILMS-TUMOR PROTEIN WT1 IS ABROGATED BY CYCLIN/CDK COMPLEXES
KUDOH, T
ISHIDATE, T
MORIYAMA, M
TOYOSHIMA, K
AKIYAMA, T
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1995, 92 (10) : 4517 - 4521
[18] Maniatis T., 1982, MOL CLONING
[19] THE UBIQUITIN-PROTEASOME PATHWAY IS REQUIRED FOR PROCESSING THE NF-KAPPA-B1 PRECURSOR PROTEIN AND THE ACTIVATION OF NF-KAPPA-B
PALOMBELLA, VJ
RANDO, OJ
GOLDBERG, AL
MANIATIS, T
[J]. CELL, 1994, 78 (05) : 773 - 785
[20] THE HPV-16 E6 AND E6-AP COMPLEX FUNCTIONS AS A UBIQUITIN-PROTEIN LIGASE IN THE UBIQUITINATION OF P53
SCHEFFNER, M
HUIBREGTSE, JM
VIERSTRA, RD
HOWLEY, PM
[J]. CELL, 1993, 75 (03) : 495 - 505

← 1 2 3 →