Cereal DNA: A rapid high-throughput extraction method for marker assisted selection

被引：34

作者：

Paris, M ^{[1
]}

Carter, M ^{[1
]}

机构：

[1] Murdoch Univ, Div Sci & Engn, Western Australian State Agr Biotechnol Ctr, Murdoch, WA 6150, Australia

来源：

PLANT MOLECULAR BIOLOGY REPORTER | 2000年 / 18卷 / 04期

关键词：

barley; cereal; DNA extraction; Hordeum; matrix mill; molecular markers; multiplex PCR; wheat;

D O I：

10.1007/BF02825063

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A rapid, automated and novel method is presented to extract DNA suitable for polymerase chain reaction (PCR) from small amounts of cereal leaf tissue in a high-throughput cost-effective way. The method uses a 96-well plate in which leaf samples are frozen, mechanically crushed using a matrix mill, macerated in alkali and subsequently neutralized. The method was used routinely with barley and wheat leaf samples and the extracted material was used to screen for specific traits of interests, including barley yellow dwarf virus resistance and beta -amylase activity in barley and stem rust resistance in wheat. This system allows complete PCR analysis of 384 seedlings or more by one person in a day.

引用

页码：357 / 360

页数：4

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