Bacillus clausii inhibits bone loss by skewing Treg-Th17 cell equilibrium in postmenopausal osteoporotic mice model

被引：69

作者：

Dar, Hamid Y. ^{[1
]}

Pal, Subhashis ^{[2
]}

Shukla, Prashant ^{[3
]}

Mishra, Pradyumna K. ^{[4
]}

Tomar, Geetanjali B. ^{[5
]}

Chattopadhyay, Naibedya ^{[2
]}

Srivastava, Rupesh K. ^{[6
]}

机构：

[1] Dr Harisingh Gour Cent Univ, Sch Biol Sci, Dept Zool, Sagar, India

[2] CDRI, Div Endocrinol, Lucknow, Uttar Pradesh, India

[3] Dr Harisingh Gour Cent Univ, Sch Math & Phys Sci, Dept Phys, Sagar, India

[4] ICMR Natl Inst Res Environm Hlth, Dept Mol Biol, Bhopal, India

[5] Savitribai Phule Pune Univ, Inst Bioinformat & Biotechnol, Pune, Maharashtra, India

[6] AIIMS, Dept Biotechnol, New Delhi 110029, India

来源：

NUTRITION | 2018年 / 54卷

关键词：

Probiotics; Bacillus clausii; Bone mass; Treg cells; Th17; cells; Osteoporosis; REGULATORY T-CELLS; MINERAL DENSITY; RHEUMATOID-ARTHRITIS; CROSS-TALK; IFN-GAMMA; OSTEOCLASTOGENESIS; PROBIOTICS; IL-17; TH17; MACROPHAGES;

D O I：

10.1016/j.nut.2018.02.013

中图分类号：

R15 [营养卫生、食品卫生]; TS201 [基础科学];

学科分类号：

100403 [营养与食品卫生学];

摘要：

Objectives: Postmenopausal osteoporosis is one of most commonly occurring skeletal diseases leading to bone loss and fragility. Probiotics have been associated with various immunomodulatory properties and thus can be exploited to enhance bone health. In the present study, we report, to our knowledge for the first time, that oral administration of Bacillus clausii (BC) in postmenopausal osteoporotic (OVX) mice model enhances bone health. Methods: BC was selected as probiotic of choice due to its established immunomodulatory properties. BC skews the Treg-Th17 cell balance in vivo by inhibiting osteoclastogenic Th17 cells and promoting antiosteoclastogenic Treg cell development in postmenopausal osteoporotic mice. Mice were divided into three groups (sham, OVX, and OVX + BC), and BC was administered orally in drinking water for 6 wk post-ovariectomy. At the end of experiment, mice were sacrificed and bones were analyzed for various parameters, along with lymphoid tissues for Treg-Th17 cells and serum cytokines. Results: We observed that BC administration enhanced bone health. This effect of BC administration was found due to skewing of Treg-Th17 cell balance (enhanced Treg and decreased Th17 cells) in vivo. BC administration reduced levels of proinflammatory cytokines (interleukin [IL]-6, IL-17, IFN-gamma and tumor necrosis factor-alpha) and increased levels of anti-inflammatory cytokine (IL-10). Conclusions: The present study strongly supports and establishes the osteoprotective potential of BC leading to enhanced bone health in postmenopausal osteoporotic mice model. (C) 2018 Elsevier Inc. All rights reserved.

引用

页码：118 / 128

页数：11

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