Functional analysis of AeSCP-2 using gene expression knockdown in the yellow fever mosquito, Aedes aegypti

被引：45

作者：

Blitzer, EJ ^{[1
]}

Vyazunova, I ^{[1
]}

Lan, Q ^{[1
]}

机构：

[1] Univ Wisconsin, Dept Entomol, Madison, WI 53706 USA

来源：

INSECT MOLECULAR BIOLOGY | 2005年 / 14卷 / 03期

关键词：

sterol carrier protein-2; RNAi; fertility; adult development; cholesterol;

D O I：

10.1111/j.1365-2583.2005.00560.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The effect of gene expression knockdown was used to study the function of the sterol carrier protein-2 (AeSCP-2) in the yellow fever mosquito, Aedes aegypti. Injection of small double stranded AeSCP-2 RNAs into mosquito larvae resulted in the knockdown of gene products. The lack of AeSCP-2 in larvae coincided with a reduction in accumulated cholesterol in pupae, supporting the hypothesis that AeSCP-2 may be involved in cholesterol uptake in mosquito larvae. Knockdown of AeSCP-2 caused a high mortality rate in developing adult and reduced egg viability. Results from this study indicate that AeSCP-2 is important for adult development and for the viability of the eggs.

引用

收藏

页码：301 / 307

页数：7

相关论文

共 34 条

[21] CULTURED AEDES-ALBOPICTUS MOSQUITO CELLS SYNTHESIZE HORMONE-INDUCIBLE PROTEINS [J].

LAN, Q ;

GERENDAY, A ;

FALLON, AM .

IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL, 1993, 29A (10) :813-818

[22] LIPID DIGESTION IN THE TSETSE-FLY, GLOSSINA-MORSITANS [J].

LANGLEY, PA ;

OGWAL, LM ;

FELTON, T ;

STAFFORD, K .

JOURNAL OF INSECT PHYSIOLOGY, 1987, 33 (12) :981-986

[23] Sterol carrier protein-2 expression in mouse L-cell fibroblasts alters cholesterol uptake [J].

Moncecchi, D ;

Murphy, EJ ;

Prows, DR ;

Schroeder, F .

BIOCHIMICA ET BIOPHYSICA ACTA-LIPIDS AND LIPID METABOLISM, 1996, 1302 (02) :110-116

[24] Partitioning of glycogen, lipid, and sugar in ovaries and body remnants of female Aedes aegypti (Diptera: Culicidae) fed human blood [J].

Naksathit, AT ;

Edman, JD ;

Scott, TW .

JOURNAL OF MEDICAL ENTOMOLOGY, 1999, 36 (01) :18-22

[25] Sterol utilization and metabolism by Heliothis zea [J].

Nes, WD ;

Lopez, M ;

Zhou, W ;

Guo, DA ;

Dowd, PF ;

Norton, RA .

LIPIDS, 1997, 32 (12) :1317-1323

[26] STEROLS IN LAODELPHAX-STRIATELLUS WITH SPECIAL REFERENCE TO THE UNICELLULAR YEASTLIKE SYMBIOTES AS A STEROL SOURCE [J].

NODA, H ;

WADA, K ;

SAITO, T .

JOURNAL OF INSECT PHYSIOLOGY, 1979, 25 (05) :443-447

[27]

Riddiford L.M., 1979, TCA (TISSUE CULTURE ASSOCIATION) MANUAL, V5, P975, DOI 10.1007/BF00919715

[28] THE EFFECTS OF CHOLESTEROL ON THE DEVELOPMENT OF HELIOTHIS-ZEA [J].

RITTER, KS ;

NES, WR .

JOURNAL OF INSECT PHYSIOLOGY, 1981, 27 (03) :175-181

[29] Recent advances in membrane microdomains: Rafts, caveolae, and intracellular cholesterol trafficking [J].

Schroeder, F ;

Gallegos, AM ;

Atshaves, BP ;

Storey, SM ;

McIntosh, AL ;

Petrescu, AD ;

Huang, H ;

Starodub, O ;

Chao, H ;

Yang, HQ ;

Frolov, A ;

Kier, AB .

EXPERIMENTAL BIOLOGY AND MEDICINE, 2001, 226 (10) :873-890

[30]

Sviridov D, 1999, HISTOL HISTOPATHOL, V14, P305, DOI 10.14670/HH-14.305

← 1 2 3 4 →