The protein of a new gene, Tctex4, interacts with protein kinase CK2β subunit and is highly expressed in mouse testis

被引：9

作者：

Bai, XY ^{[1
]}

Xu, X

Zhang, FY

Shu, HB

Chan, ED

机构：

[1] McLaughlin Res Inst Biomed Sci, Great Falls, MT 59405 USA

[2] Natl Jewish Med & Res Ctr, Dept Med, Denver, CO 80206 USA

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2003年 / 307卷 / 01期

关键词：

protein kinase CK2; Tctex4; protein-protein interaction; testis;

D O I：

10.1016/S0006-291X(03)01118-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Casein kinase 2 (CK2) is a ubiquitous, multifunctional eukaryotic serine/threonine kinase that phosphorylates an array of proteins. CK2 is a heterotetramer composed of two catalytic (alpha, alpha') and two regulatory (beta) subunits. CK2 plays an essential role in regulatory pathways in cell transformation and proliferation. But the role and function of the individual subunits of CK2. which are not in the holoenzyme, are not yet clear. Northern blot analysis reveals the highest CK2beta activity in mouse testicles and brain. By employing a yeast two-hybrid screen to identify the proteins that interact with CK2beta, we have isolated a cDNA clone encoding a 14-kDa protein with homology to dynein light chains and have designated it as Tctex4. CK2beta interacts specifically with Tctex4 both in a yeast two-hybrid system and in an in vitro interaction assay. Northern blot and in situ hybridization showed that Tctex4 is a novel gene that is expressed in mouse testis. (C) 2003 Elsevier Science (USA). All rights reserved.

引用

页码：86 / 91

页数：6

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