Effects of lead nitrate and sodium selenite on DNA damage and oxidative stress in diabetic and non-diabetic rat erythrocytes and leucocytes

被引：25

作者：

Bas, Hatice ^{[1
]}

Kalender, Yusuf ^{[2
]}

Pandir, Dilek ^{[1
]}

Kalender, Suna ^{[3
]}

机构：

[1] Bozok Univ, Fac Arts & Sci, Dept Biol, TR-66100 Yozgat, Turkey

[2] Gazi Univ, Fac Sci, Dept Biol, TR-06500 Ankara, Turkey

[3] Gazi Univ, Gazi Educ Fac, Dept Sci Educ, TR-06500 Ankara, Turkey

来源：

ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY | 2015年 / 39卷 / 03期

关键词：

Lead; Selenium; Diabetes; Oxidative stress; Antioxidant enzyme; DNA damage; ANTIOXIDANT ACTIVITY; GLUTATHIONE-PEROXIDASE; VITAMIN-E; TISSUES; ASSAY; ACID; INVOLVEMENT; QUERCETIN; TOXICITY; EXTRACT;

D O I：

10.1016/j.etap.2015.03.012

中图分类号：

X [环境科学、安全科学];

学科分类号：

083001 [环境科学];

摘要：

The adverse effects of lead nitrate (LN) and the preventive role of sodium selenite were investigated in diabetic and non-diabetic rat blood by measuring trolox equivalent antioxidant capacity (TEAC), ferric reducing antioxidant power (FRAP), malondialdehyde (MDA) levels and activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and glutathione-S-transferase (GST) also by evaluating DNA damage with comet assay. LN increased the levels of MDA, tail DNA%, mean tail length and tail moment, decreased the enzymes activities, FRAP and TEAC values. In sodium selenite +LN group, we observed the protective effect of sodium selenite on examining parameters. Diabetes caused alterations on these parameters, too. We found that sodium selenite did not protect against diabetes caused damages. As a result, LN caused toxic effects on blood cells and sodium selenite alleviated this toxicity but it did not show preventive effect against diabetes. Also, LN caused more harmfull effects in diabetic groups than non-diabetic groups. (C) 2015 Elsevier B.V. All rights reserved.

引用

页码：1019 / 1026

页数：8

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