Efficient transformation of filamentous fungus Pleurotus ostreatus using single-strand carrier DNA

被引：41

作者：

Irie, T ^{[1
]}

Honda, Y ^{[1
]}

Watanabe, T ^{[1
]}

Kuwahara, M ^{[1
]}

机构：

[1] Kyoto Univ, Wood Res Inst, Kyoto 6110011, Japan

来源：

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY | 2001年 / 55卷 / 05期

关键词：

D O I：

10.1007/s002530000535

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The effects of carrier DNAs on the transformation of the basidiomycete Pleurotus ostreatus were analyzed. When lambda phage DNA was added to a transformation mixture containing protoplasts and Cbx(R) vector plasmid, an increased number of drug-resistant transformants was observed on a screening plate containing 2 mug carboxin/ml. The highest efficiency (about 200 transformants/mug Vector plasmid) was obtained by the addition of heat-denatured lambda DNA, which gave yields approximately 50-fold higher than the control experiment without a carrier DNA. To our knowledge, this is the first report on enhancement in transformation efficiency of fungal protoplasts by single strand carrier DNA.

引用

页码：563 / 565

页数：3

共 19 条

[1] STRATEGIES FOR HIGH-EFFICIENCY COTRANSFORMATION OF NEUROSPORA-CRASSA
AUSTIN, B
TYLER, BM
[J]. EXPERIMENTAL MYCOLOGY, 1990, 14 (01): : 9 - 17
[2] Initial oxidation products in the metabolism of pyrene, anthracene, fluorene, and dibenzothiophene by the white rot fungus Pleurotus ostreatus
Bezalel, L
Hadar, Y
Fu, PP
Freeman, JP
Cerniglia, CE
[J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1996, 62 (07) : 2554 - 2559
[3] Metabolism of phenanthrene by the white rot fungus Pleurotus ostreatus
Bezalel, L
Hadar, Y
Fu, PP
Freeman, JP
Cerniglia, CE
[J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1996, 62 (07) : 2547 - 2553
[4] BYUN M-O, 1989, Korean Journal of Mycology, V17, P209
[5] BYUN M-O, 1989, Korean Journal of Mycology, V17, P27
[6] Agrobacterium tumefaciens-mediated transformation of filamentous fungi
de Groot, MJA
Bundock, P
Hooykaas, PJJ
Beijersbergen, AGM
[J]. NATURE BIOTECHNOLOGY, 1998, 16 (09) : 839 - 842
[7] STUDIES ON THE TRANSFORMATION OF INTACT YEAST-CELLS BY THE LIAC/S-DNA/PEG PROCEDURE
GIETZ, RD
SCHIESTL, RH
WILLEMS, AR
WOODS, RA
[J]. YEAST, 1995, 11 (04) : 355 - 360
[8] Carboxin resistance transformation of the homobasidiomycete fungus Pleurotus ostreatus
Honda, Y
Matsuyama, T
Irie, T
Watanabe, T
Kuwahara, M
[J]. CURRENT GENETICS, 2000, 37 (03) : 209 - 212
[9] Transformation of the edible fungi, Pleurotus ostreatus and Volvariella volvacea
Jia, JH
Buswell, JA
Peberdy, JF
[J]. MYCOLOGICAL RESEARCH, 1998, 102 : 876 - 880
[10] Kim BG, 1999, FEMS MICROBIOL LETT, V181, P225, DOI 10.1111/j.1574-6968.1999.tb08848.x

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